Detection of beryllium sensitivity using a flow cytometric lymphocyte proliferation test: the Immuno-Be-LPT

被引：25

作者：

Farris, GM

Newman, LS

Frome, EL

Shou, YL

Barker, E

Habbersett, RC

Maier, L

Smith, HN

Marrone, BL

机构：

[1] Univ Calif Los Alamos Natl Lab, Cytometry Grp, BioSci Div, Los Alamos, NM 87545 USA

[2] Natl Jewish Med & Res Ctr, Div Environm & Occupat Hlth Sci, Denver, CO 80206 USA

[3] Oak Ridge Natl Lab, Div Math & Comp Sci, Oak Ridge, TN 37831 USA

[4] Univ Calif Los Alamos Natl Lab, Environm Safety & Hlth Div, Los Alamos, NM 87545 USA

来源：

TOXICOLOGY | 2000年 / 143卷 / 02期

关键词：

beryllium; lymphocyte proliferation test; flow cytometry; autologous plasma; CD4; CD8; immunophenotyping; hypersensitivity; BrdU; DAPI; PI; tetanus; chronic beryllium disease;

D O I：

10.1016/S0300-483X(99)00167-5

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Measurement of lymphocyte proliferation to detect hypersensitivity to beryllium (Be-LPT) in vitro is done presently using a method based on tritiated thymidine incorporation. Although this method is sensitive it gives no information on cell viability or responding lymphocyte subsets. We have developed reliable and simple flow cytometric assays for lymphocyte proliferation testing (Immuno-Be-LPT) by combining immunophenotyping with bromodeoxyuridine (BrdU) incorporation or DNA content using propidium iodide (PI) or 4'6'-diimidazolin-2-phenylindole (DAPI), Evaluation of beryllium-induced lymphocyte proliferation in blood cells from seven patients with chronic beryllium disease (CBD) and 120 beryllium workers by both the Be-LPT and the Immuno-Be-LPT showed agreement between the tests. The Immuno-Be-LPT provided additional information about the specific type of lymphocytes responding. CD4+ lymphocytes proliferated in response to beryllium in blood samples from all seven CBD individuals and CD8+ lymphocytes proliferated in six of the seven. Four beryllium workers without CBD had positive responses to beryllium primarily in the CD8+ cells. The use of the individual's own plasma supported a greater beryllium or tetanus-induced proliferation of CD4+ lymphocytes when compared to commercial human serum. The response of CD4+ lymphocytes measured in the Immuno-Be-LPT may provide a new marker for the diagnosis of CBD. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：125 / 140

页数：16

共 25 条

[1] A flow cytometric method to assess antigen-specific proliferative responses of different subpopulations of fresh and cryopreserved human peripheral blood mononuclear cells [J].