Single cell trap on a chip using in-situ microfabrication with photo-crosslinkable resin and thermal gelation

被引：6

作者：

Ichikawa, A ^{[1
]}

Arai, F ^{[1
]}

Maruyama, H ^{[1
]}

Fukuda, T ^{[1
]}

Katsuragi, T ^{[1
]}

机构：

[1] Nagoya Univ, Dept Micro Syst Engn, Chikusa Ku, Nagoya, Aichi 4648603, Japan

来源：

2004 IEEE INTERNATIONAL CONFERENCE ON ROBOTICS AND AUTOMATION, VOLS 1- 5, PROCEEDINGS | 2004年

关键词：

micromanipulation; culture; separation; microorganisms; photo-crosslinkable resin; thermal gelation; mu-TAS;

D O I：

10.1109/ROBOT.2004.1307492

中图分类号：

TP [自动化技术、计算机技术];

学科分类号：

0812 ;

摘要：

Handling of microorganisms is of great interests in biology and biotechnology. Single cell capture technology has been demonstrated in our previous work with thermal gelation material and micro fluidic devise. This paper extends the system by local flow rate control to stabilize the trap with in-situ fabrication by photo-crosslinkable resin. Target cells are captured by gel and enclosed with the micro-structure. The cells, therefore, are protected from high-speed-channel flow or disturbances. This system succeeds in the separation and continuous culture of yeast cells, reaction and monitoring of the cultured cells stably by fluorescence staining in situ on a chip.

引用

页码：2848 / 2853

页数：6

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