Susceptibility of nontypeable haemophilus influenzae to human β-defensins is influenced by lipooligosaccharide acylation

被引：60

作者：

Starner, TD

Swords, WE

Apicella, MA

McCray, PB

机构：

[1] Univ Iowa, Dept Pediat, Iowa City, IA 52242 USA

[2] Univ Iowa, Dept Microbiol, Iowa City, IA 52242 USA

来源：

INFECTION AND IMMUNITY | 2002年 / 70卷 / 09期

关键词：

D O I：

10.1128/IAI.70.9.5287-5289.2002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Nontypeable Haemophilus influenzae (NTHI) lipooligosaccharide htrB mutants exhibited greater than 45-fold-increased sensitivity to human beta-defensin 2 (HBD-2) compared to the wild type. Complementation by htrB in trans to acylation competence reversed this increased sensitivity. In contrast, NTHI was more susceptible to HBD-3 and showed no changes in sensitivity as a result of lipooligosaccharide mutations in oligosaccharide and lipid A biosynthesis genes.

引用

收藏

页码：5287 / 5289

页数：3

相关论文

共 26 条

[1] Evaluation of the virulence of nontypeable Haemophilus influenzae lipooligosaccharide htrB and rfaD mutants in the chinchilla model of otitis media [J].

DeMaria, TF ;

Apicella, MA ;

Nichols, WA ;

Leake, ER .

INFECTION AND IMMUNITY, 1997, 65 (11) :4431-4435

[2] Specific lipopolysaccharide found in cystic fibrosis airway Pseudomonas aeruginosa [J].

Ernst, RK ;

Yi, EC ;

Guo, L ;

Lim, KB ;

Burns, JL ;

Hackett, M ;

Miller, SI .

SCIENCE, 1999, 286 (5444) :1561-1565

[3] Lipid A acylation and bacterial resistance against vertebrate antimicrobial peptides [J].

Guo, L ;

Lim, KB ;

Poduje, CM ;

Daniel, M ;

Gunn, JS ;

Hackett, M ;

Miller, SI .

CELL, 1998, 95 (02) :189-198

[4] Isolation and characterization of human β-defensin-3, a novel human inducible peptide antibiotic [J].

Harder, J ;

Bartels, J ;

Christophers, E ;

Schröder, JM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (08) :5707-5713

[5] A peptide antibiotic from human skin [J].

Harder, J ;

Bartels, J ;

Christophers, E ;

Schroder, JM .

NATURE, 1997, 387 (6636) :861-861

[6] Sialic acid in the lipopolysaccharide of Haemophilus influenzae:: strain distribution, influence on serum resistance and structural characterization [J].

Hood, DW ;

Makepeace, K ;

Deadman, ME ;

Rest, RF ;

Thibault, P ;

Martin, A ;

Richards, JC ;

Moxon, ER .

MOLECULAR MICROBIOLOGY, 1999, 33 (04) :679-692

[7] The structure of human β-defensin-2 shows evidence of higher order oligomerization [J].

Hoover, DM ;

Rajashankar, KR ;

Blumenthal, R ;

Puri, A ;

Oppenheim, JJ ;

Chertov, O ;

Lubkowski, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (42) :32911-32918

[8] International Economic Law in times that are interesting [J].

Jackson, JH .

JOURNAL OF INTERNATIONAL ECONOMIC LAW, 2000, 3 (01) :3-14

[9]

Jia HP, 2001, GENE, V263, P211

[10] MUTATION OF THE HTRB LOCUS OF HAEMOPHILUS-INFLUENZAE NONTYPABLE STRAIN-2019 IS ASSOCIATED WITH MODIFICATIONS OF LIPID-A AND PHOSPHORYLATION OF THE LIPO-OLIGOSACCHARIDE [J].

LEE, NG ;

SUNSHINE, MG ;

ENGSTROM, JJ ;

GIBSON, BW ;

APICELLA, MA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (45) :27151-27159