Photochemically induced gene silencing using PNA-peptide conjugates

被引：18

作者：

Boe, S. ^{[1
]}

Hovig, E. ^{[1
]}

机构：

[1] Norwegian Radium Hosp, Dept Tumor Biol, Inst Canc Res, NO-0310 Oslo, Norway

来源：

OLIGONUCLEOTIDES | 2006年 / 16卷 / 02期

关键词：

D O I：

10.1089/oli.2006.16.145

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The potential for exploration of peptide nucleic acid (PNA) as an experimental and therapeutic regulator of gene expression has been hampered by a poor delivery and a lack of site-specific targeting. In the present study, we have developed an efficient strategy for nuclear delivery of PNA by combining cationically charged PNA-peptide conjugates and photochemical internalization (PCI) technology. When using the S100A4 gene as a model system, a consistent downregulation to around 10% remaining protein signal was obtained in three selected cell lines. Furthermore, a dose-dependent and time-dependent inhibition of the S100A4 protein was demonstrated. A main benefit of the strategy proposed is the possibility of site-specific targeting.

引用

页码：145 / 157

页数：13

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