Diversity of the Enzymatic Activity in the Lipoxygenase Gene Family of Arabidopsis thaliana

被引：199

作者：

Bannenberg, Gerard ^{[1
]}

Martinez, Marta ^{[1
]}

Hamberg, Mats ^{[2
]}

Castresana, Carmen ^{[1
]}

机构：

[1] CSIC, Ctr Nacl Biotecnol, Dept Genet Mol Plantas, Madrid, Spain

[2] Karolinska Inst, Dept Med Biochem & Biophys, Div Physiol Chem 2, Stockholm, Sweden

来源：

LIPIDS | 2009年 / 44卷 / 02期

基金：

瑞典研究理事会;

关键词：

Arabidopsis thaliana; Fatty acid; Fatty acid hydroperoxide; Lipoxygenase; Oxylipins; JASMONIC ACID; POTATO LIPOXYGENASE; DEFENSE RESPONSES; PATHWAY; RESISTANCE; OXYLIPINS; BIOSYNTHESIS; INVOLVEMENT; PATHOGENS; SIGNAL;

D O I：

10.1007/s11745-008-3245-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipoxygenases (LOX) catalyze the oxygenation of polyunsaturated fatty acids, the first step in the biosynthesis of a large group of biologically active fatty acid metabolites collectively named oxylipins. In the present study we report the characterization of the enzymatic activity of the six lipoxygenases found in the genome of the model plant Arabidopsis thaliana. Recombinant expressed AtLOX-1 and AtLOX-5 had comparable oxygenase activity with either linoleic acid or linolenic acid. AtLOX-2, AtLOX-3, AtLOX-4 and AtLOX-6 displayed a selective oxygenation of linolenic acid. Analyses by high-performance liquid chromatography and gas chromatography-mass spectrometry demonstrated that AtLOX-1 and AtLOX-5 are 9S-lipoxygenases, and AtLOX-2, AtLOX-3, AtLOX-4 and AtLOX-6 are 13S-lipoxygenases. None of the enzymes had dual positional specificity. The determined activities correlated with that predicted by their phylogenetic relationship to other biochemically-characterized plant lipoxygenases.

引用

页码：85 / 95

页数：11

共 48 条

[1] CHARACTERIZATION OF AN ARABIDOPSIS-LIPOXYGENASE GENE RESPONSIVE TO METHYL JASMONATE AND WOUNDING [J].

BELL, E ;

MULLET, JE .

PLANT PHYSIOLOGY, 1993, 103 (04) :1133-1137

[2] A CHLOROPLAST LIPOXYGENASE IS REQUIRED FOR WOUND-INDUCED JASMONIC ACID ACCUMULATION IN ARABIDOPSIS [J].

BELL, E ;

CREELMAN, RA ;

MULLET, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (19) :8675-8679

[3] Enzymatic and non-enzymatic lipid peroxidation in leaf development [J].

Berger, S ;

Weichert, H ;

Porzel, A ;

Wasternack, C ;

Kühn, H ;

Feussner, I .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2001, 1533 (03) :266-276

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] Lipoxygenases: Occurrence, functions, catalysis, and acquisition of substrate [J].

Brash, AR .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (34) :23679-23682

[6] Reciprocal oxylipin-mediated cross-talk in the Aspergillus-seed pathosystem [J].

Brodhagen, Marion ;

Tsitsigiannis, Dimitrios I. ;

Hornung, Ellen ;

Goebel, Cornelia ;

Feussner, Ivo ;

Keller, Nancy P. .

MOLECULAR MICROBIOLOGY, 2008, 67 (02) :378-391

[7] Jasmonate: An oxylipin signal with many roles in plants [J].

Browse, J .

PLANT HORMONES, 2005, 72 :431-456

[8] Distinct Roles of Jasmonates and Aldehydes in Plant-Defense Responses [J].

Chehab, E. Wassim ;

Kaspi, Roy ;

Savchenko, Tatyana ;

Rowe, Heather ;

Negre-Zakharov, Florence ;

Kliebenstein, Dan ;

Dehesh, Katayoon .

PLOS ONE, 2008, 3 (04)

[9] The JAZ family of repressors is the missing link in jasmonate signalling [J].

Chini, Andrea ;

Fonseca, S. ;

Fernandez, G. ;

Adie, B. ;

Chico, J. M. ;

Lorenzo, O. ;

Garcia-Casado, G. ;

Lopez-Vidriero, I. ;

Lozano, F. M. ;

Ponce, M. R. ;

Micol, J. L. ;

Solano, R. .

NATURE, 2007, 448 (7154) :666-+

[10] Regulation and function of Arabidopsis JASMONATE ZIM-domain genes in response to wounding and herbivory [J].

Chung, Hoo Sun ;

Koo, Abraham J. K. ;

Gao, Xiaoli ;

Jayanty, Sastry ;

Thines, Bryan ;

Jones, A. Daniel ;

Howe, Gregg A. .

PLANT PHYSIOLOGY, 2008, 146 (03) :952-964

← 1 2 3 4 5 →