Correlated expression of human beta defensin-1, -2 and -3 mRNAs in gingival tissues of young children

被引：18

作者：

Saitoh, M

Abiko, Y ^{[1
]}

Shimabukuro, S

Kusano, K

Nishimura, M

Arakawa, T

Nakashima, K

Takuma, T

Kaku, T

Igarashi, S

机构：

[1] Hlth Sci Univ Hokkaido, Dept Oral Pathol, Ishikari, Hokkaido 0610293, Japan

[2] Hlth Sci Univ Hokkaido, Dept Pediat Dent, Ishikari, Hokkaido 0610293, Japan

[3] Hlth Sci Univ Hokkaido, Dept Oral Biochem, Ishikari, Hokkaido 0610293, Japan

[4] Hlth Sci Univ Hokkaido, Dept Periodontol, Ishikari, Hokkaido 0610293, Japan

来源：

ARCHIVES OF ORAL BIOLOGY | 2004年 / 49卷 / 10期

关键词：

defensin; gingiva; RT-PCR; antimicrobial peptide;

D O I：

10.1016/j.archoralbio.2004.04.012

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Objective: Human beta-defensins (hBDs) are a group of antimicrobial peptides, expressed by the epithelial. cells of many organs including gingival epithelium. The present study examined correlation between the gene expressions of hBD-1, -2, -3 mRNAs and the inflammatory cytokines in human gingival tissues. Study design: The gingival. tissues were obtained from surgical discards from 20 different patients (age range, 5-13 years). The expression levels of mRNAs were evaluated by quantitative RT- PCR with LightCycler(TM). The mRNA expression levels were normalized with those of keratin 10 mRNA. The data were statistically analysed using Person's correlation coefficient. Results: The expression levels of hBD-1,-2 and -3 were significantly correlated with each other and also correlated with that of TNF-alpha. Conclusions: The results indicate that the expression levels of hBDs vary from one individual to another. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：799 / 803

页数：5

共 22 条

[1] Differential expression of human beta-defensin 2 in keratinized and non-keratinized oral epithelial lesions; immunohistochemistry and in situ hybridization [J].