Actin -: an inhibitor of eukaryotic elongation factor activities

被引：15

作者：

Bektas, M ^{[1
]}

Günçer, B ^{[1
]}

Güven, C ^{[1
]}

Nurten, R ^{[1
]}

Bermek, E ^{[1
]}

机构：

[1] Istanbul Univ, Istanbul Fac Med, Dept Biophys, TR-34390 Istanbul, Turkey

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 317卷 / 04期

关键词：

eukaryotic elongation factors 1 and 2; actin; cytoskeleton; ADP-ribosylation; protein synthesis;

D O I：

10.1016/j.bbrc.2004.03.153

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An inhibitor of diphtheria toxin- and endogenous transferase-dependent ADP-ribosylation of eukaryotic elongation factor 2 (eEF2) has been found in the cytoplasmic fraction from rat liver. We provide evidence that this cytoplasmic inhibitor corresponds to actin, which gives rise also to inhibition of polyphenylalanine (polyPhe) synthesis. Both globular monomeric (G-actin) and filamentous (F-actin) forms of actin appear to be inhibitory on the action of elongation factors 1 and 2 (eEF1 and eEF2) in polyPhe synthesis with the inhibitory effect of G-actin proving to be stronger. Some component(s) in the postribosomal supernatant (S-130) fraction and also DNase I prevent actin-promoted inhibition of polyPhe synthesis. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：1061 / 1066

页数：6

共 31 条

[1] Interactions of elongation factor 2 with the cytoskeleton and interference with DNase I binding to actin [J].

Bektas, M ;

Nurten, R ;

Sayers, Z ;

Bermek, E .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1998, 256 (01) :142-147

[2] INTERACTIONS OF EUKARYOTIC ELONGATION-FACTOR-2 WITH ACTIN - A POSSIBLE LINK BETWEEN PROTEIN SYNTHETIC MACHINERY AND CYTOSKELETON [J].

BEKTAS, M ;

NURTEN, R ;

GUREL, Z ;

SAYERS, Z ;

BERMEK, E .

FEBS LETTERS, 1994, 356 (01) :89-93

[3]

Bermek E, 1978, Prog Nucleic Acid Res Mol Biol, V21, P63, DOI 10.1016/S0079-6603(08)60267-6

[4] BINDING OF PERIODATE-OXIDIZED GUANINE-NUCLEOTIDES TO EUKARYOTIC ELONGATION FACTOR-II [J].

BILGIN, N ;

SAYHAN, O ;

BERMEK, E .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1048 (2-3) :217-222

[5]

CHANG MP, 1989, J BIOL CHEM, V264, P15261

[6] Regulating actin-filament dynamics in vivo [J].

Chen, H ;

Bernstein, BW ;

Bamburg, JR .

TRENDS IN BIOCHEMICAL SCIENCES, 2000, 25 (01) :19-23

[7] EFFECT OF DIPHTHERIA TOXIN ON PROTEIN SYNTHESIS - INACTIVATION OF 1 OF TRANSFER FACTORS [J].

COLLIER, RJ .

JOURNAL OF MOLECULAR BIOLOGY, 1967, 25 (01) :83-&

[8] DIPHTHERIA-TOXIN - MODE OF ACTION AND STRUCTURE [J].

COLLIER, RJ .

BACTERIOLOGICAL REVIEWS, 1975, 39 (01) :54-85

[9] ELONGATION-FACTOR 1-ALPHA, TRANSLATION AND THE CYTOSKELETON [J].

CONDEELIS, J .

TRENDS IN BIOCHEMICAL SCIENCES, 1995, 20 (05) :169-170

[10] Moonlighting functions of polypeptide elongation factor 1: From actin bundling to zinc finger protein R1-associated nuclear localization [J].

Ejiri, S .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2002, 66 (01) :1-21

← 1 2 3 4 →