Biomonitoring of antimony in environmental matrices from terrestrial and limnic ecosystems

Elder and poplar leaves from various sampling sites were studied with respect to their antimony content. Moreover, a retrospective determination of Sb was performed in representative limnic and terrestrial samples of the Federal Enviromnental Specimen Bank of Germany which have been collected over 14 years. The analytical procedure is based on an open vessel acid digestion of freeze-dried biological samples and the subsequent quantification of Sb in the digests by flow injection hydride generation atomic absorption spectrometry. Strict quality control schemes were applied to the entire procedure to guarantee accurate and precise results. No long-term changes of the Sb concentrations were found in spruce shoots or poplar leaves from different sampling sites. However, spruce shoots from a semi-natural region showed concentrations of Sb (similar to 22 ng g(-1); range: 17-29 ng g(-1)) that were approximately four times lower than in corresponding samples from an urban-industrialized area. The analysis of virgin and washed elder leaves revealed that approximately 20-30% of the Sb is present on the leaf surface. Sb levels in elder leaves ranged from 5.2 +/- 0.3 ng g(-1) in samples from Argentina to 589 +/- 30 ng g(-1) in leaves collected directly beside a motorway in Germany. Similarly, poplar leaves from Argentina and Chile showed about 4 ng Sb g(-1) whereas 150 ng Sb g(-1) was found in poplar leaves from Germany. The lowest concentrations of Sb were determined in digests of pigeon eggs (similar to 2 ng g(-1)), bream liver (similar to 4 ng g(-1)) and deer liver (similar to 6 ng g(-1)). A similar pattern of Sb concentrations in spruce shoots, leaves or liver samples from an industrialized area and an agrarian ecosystem in Germany was established. Concentrations of Sb in elder leaves were closely associated with car traffic, giving maximum concentrations of 589 ng g(-1) directly beside a motorway, 207 ng g(-1) 50 m from the motorway and 153 ng g(-1) in a dose residential area.