A novel strategy for selection of allosteric ribozymes yields RiboReporter™ sensors for caffeine and aspartame

被引：28

作者：

Ferguson, A ^{[1
]}

Boomer, RM ^{[1
]}

Kurz, M ^{[1
]}

Keene, SC ^{[1
]}

Diener, JL ^{[1
]}

Keefe, AD ^{[1
]}

Wilson, C ^{[1
]}

Cload, ST ^{[1
]}

机构：

[1] Archemix Corp, Cambridge, MA 02139 USA

来源：

NUCLEIC ACIDS RESEARCH | 2004年 / 32卷 / 05期

关键词：

D O I：

10.1093/nar/gkh336

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have utilized in vitro selection technology to develop allosteric ribozyme sensors that are specific for the small molecule analytes caffeine or aspartame. Caffeine- or aspartame-responsive ribozymes were converted into fluorescence-based RiboReporter(TM) sensor systems that were able to detect caffeine or aspartame in solution over a concentration range from 0.5 to 5 mM. With read-times as short as 5 min, these caffeine- or aspartame-dependent ribozymes function as highly specific and facile molecular sensors. Interestingly, successful isolation of allosteric ribozymes for the analytes described here was enabled by a novel selection strategy that incorporated elements of both modular design and activity-based selection methods typically used for generation of catalytic nucleic acids.

引用

页码：1756 / 1766

页数：11

共 31 条

[1] A SIMPLE METHOD FOR THE ESTIMATION OF AMINO-GROUPS ON INSOLUBLE MATRIX BEADS [J].