Gene expression changes during mouse skeletal myoblast differentiation revealed by transcriptional profiling

被引：91

作者：

Moran, JL ^{[1
]}

Li, YZ ^{[1
]}

Hill, AA ^{[1
]}

Mounts, WM ^{[1
]}

Miller, CP ^{[1
]}

机构：

[1] Wyeth Res, Dept Genom, Cambridge, MA 02140 USA

来源：

PHYSIOLOGICAL GENOMICS | 2002年 / 10卷 / 02期

关键词：

C2C12; cells; oligonucleotide array; functional category enrichment;

D O I：

10.1152/physiolgenomics.00011.2002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 [细胞生物学]; 090102 [作物遗传育种];

摘要：

Studies described here utilize high-density oligonucleotide arrays to characterize changes in global mRNA expression patterns during proliferation, cell cycle withdrawal, and terminal differentiation in mouse C2C12 myoblasts. Statistical analyses revealed 629 sequences differentially regulated between proliferating and differentiating myoblasts. These genes were clustered using self-organizing maps to identify sets of coregulated genes and were assigned to functional categories that were analyzed for distribution across expression clusters. Clusters were identified with statistically significant enrichment of functional categories including muscle contraction, cell adhesion, extracellular matrix function, cellular metabolism, mitochondrial transport, DNA replication, cell cycle control, mRNA transcription, and unexpectedly, immune regulation. In addition, functional category enrichment data can be used to predict gene function for numerous differentially regulated expressed sequence tags. The results provide new insight into how genes involved in these cellular processes may play a role in skeletal muscle growth and differentiation.

引用

页码：103 / 111

页数：9

共 45 条

[1]

Myogenin expression, cell cycle withdrawal, and phenotypic differentiation are temporally separable events that precede cell fusion upon myogenesis [J].