A microfabricated, electrochemiluminescence cell for the detection of amplified DNA

被引：40

作者：

Hsueh, YT

Smith, RL

Northrup, MA

机构：

[1] UNIV CALIF DAVIS,DEPT ELECT & COMP ENGN,DAVIS,CA 95616

[2] LAWRENCE LIVERMORE NATL LAB,MICROTECHNOL CTR,LIVERMORE,CA

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1996年 / 33卷 / 1-3期

关键词：

electrochemiluminescence cell; amplified DNA;

D O I：

10.1016/0925-4005(96)01933-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An electrochemical cell for the generation and detection of electrochemiluminescence of tris (2,2'-bipyridyl) ruthenium(II) has been microfabricated in silicon. The cell has been designed for use in the detection and quantification of DNA which has been amplified by the polymerase chain reaction. The cell is a vertical assembly of micromachined silicon and glass substrates, containing a gold, thin film cathode and an indium tin oxide, thin film anode. The anode is transparent, enabling the detection of the luminescence by an external photodetector. Preliminary tests have yielded the detection of free TBR concentrations from 10(-9) M to 10(-3) M with a cell volume of 85 mu l.

引用

页码：110 / 114

页数：5

共 5 条

[1]

BLACKBURN GF, 1991, CLIN CHEM, V37, P1534

[2]

DICESARE J, 1993, BIOTECHNIQUES, V15, P152

[3] DNA AMPLIFICATION BY THE POLYMERASE CHAIN-REACTION [J].