The generation of memory antigen-specific cytotoxic T cell responses by CD28/CD80 interactions in the absence of antigen

被引:18
作者
Flynn, K [1 ]
Mullbacher, A [1 ]
机构
[1] AUSTRALIAN NATL UNIV, JOHN CURTIN SCH MED RES, DIV CELL BIOL & IMMUNOL, CANBERRA, ACT 2601, AUSTRALIA
关键词
immunological memory; co-stimulation; cytotoxic T cell; bystander activation;
D O I
10.1002/eji.1830270216
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The interaction of co-stimulatory molecules CT80/CD86 on antigen-presenting cells with CD28 on naive CD8(+) cytotoxic T (Tc) cells is understood to be critical in the induction of Tc effecters. CD80 is capable of providing signal 2 for the activation of Tc cells, but has no effect if encountered in the absence of specific peptide/MHC complexes (signal 1). We have found that CD80 presented in vitro to resting memory viral-immune or alloimmune Tc cells can provide sufficient stimulus for the generation of effector Tc cells in the absence of specific antigen, the peptide/MHC class I complex. Effector Tc cells generated in vitro from influenza- or class I alloantigen-primed mice by co-stimulation in the absence of antigen require exogenous interleukin (IL)-2 signaling via the cell surface-expressed IL-2 receptor or, under conditions of IL-2 blockade, exogenous IL-7. Activation of memory Tc cells by signal 1 and 2 is independent of IL-2 and IL-7. Although memory influenza-immune Tc cells did respond to CD80 in the absence of antigen, the presence of antigen +CD80 enabled an earlier induction of these Tc cells and they retained their lytic activity in vitro over a longer time period. The capacity of memory Tc cells to be activated by signal 2 alone provides one explanation for the observed heterogeneity of phenotype of memory T cells in vivo and a possible mechanism for the maintenence of memory in the absence of persisting antigen.
引用
收藏
页码:456 / 462
页数:7
相关论文
共 55 条
[1]   RECOGNITION BY MAJOR HISTOCOMPATIBILITY COMPLEX CLASS I-RESTRICTED CYTOLYTIC LYMPHOCYTES-T OF CELLS EXPRESSING VACCINIA-ENCODED VIRAL AND CLASS-I PROTEINS [J].
ANDREW, ME ;
COUPAR, BEH ;
BOYLE, DB ;
BLANDEN, RV .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1987, 17 (10) :1515-1518
[2]   CD28 INTERACTION WITH B7-COSTIMULATES PRIMARY ALLOGENEIC PROLIFERATIVE RESPONSES AND CYTOTOXICITY MEDIATED BY SMALL, RESTING LYMPHOCYTES-T [J].
AZUMA, M ;
CAYABYAB, M ;
BUCK, D ;
PHILLIPS, JH ;
LANIER, LL .
JOURNAL OF EXPERIMENTAL MEDICINE, 1992, 175 (02) :353-360
[3]  
BAROJA ML, 1988, J IMMUNOL, V141, P1502
[4]  
BERTAGNOLLI M, 1990, J IMMUNOL, V145, P1706
[5]   IS T-CELL MEMORY MAINTAINED BY CROSS-REACTIVE STIMULATION [J].
BEVERLEY, PCL .
IMMUNOLOGY TODAY, 1990, 11 (06) :203-205
[6]   ENHANCED RECOGNITION OF A MODIFIED PEPTIDE ANTIGEN BY CYTOTOXIC-T CELLS SPECIFIC FOR INFLUENZA NUCLEOPROTEIN [J].
BODMER, HC ;
PEMBERTON, RM ;
ROTHBARD, JB ;
ASKONAS, BA .
CELL, 1988, 52 (02) :253-258
[7]  
Brinkmann V, 1996, J IMMUNOL, V156, P4100
[8]   ON THE CELLULAR BASIS OF IMMUNOLOGICAL T-CELL MEMORY [J].
BRUNO, L ;
KIRBERG, J ;
VONBOEHMER, H .
IMMUNITY, 1995, 2 (01) :37-43
[9]  
BUDD RC, 1987, J IMMUNOL, V138, P3583
[10]  
CELADA F, 1971, PROG ALLERGY, V15, P223