Monitoring and visualising plant cuticles by confocal laser scanning microscopy

被引：29

作者：

Fernández, S ^{[1
]}

Osorio, S ^{[1
]}

Heredia, A ^{[1
]}

机构：

[1] Univ Malaga, Fac Ciencias, Dept Biol Mol & Bioquim, E-29071 Malaga, Spain

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 1999年 / 37卷 / 10期

关键词：

confocal laser scanning microscopy; cuticle; cutin; flavonoids; phenolic compounds;

D O I：

10.1016/S0981-9428(00)86692-9

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The present work shows the visualisation of phenolics and flavonoids of plant cuticles by confocal laser scanning microscopy (CLSM). Selected isolated fruit and leaf cuticles were monitored on the basis of autofluorescent phenolics and flavonoids which, in most cases, permitted us to obtain three-dimensional images of the cuticular membranes. The utility of this technique in investigations of cuticular translocation and diffusion of exogenous applied chemicals and cuticle degradation has also been explored. (C) 1999 Editions scientifiques et medicales Elsevier SAS.

引用

页码：789 / 794

页数：6

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