Extravascular transport of fluorescently labeled albumins in the rat mesentery

被引:9
作者
Harris, NR
Whitt, SP
Zilberberg, J
Alexander, JS
Rumbaut, RE
机构
[1] Penn State Univ, Dept Bioengn, University Pk, PA 16802 USA
[2] Univ Missouri, Pulm & Crit Care Div, Dept Internal Med, Columbia, MO USA
[3] Louisiana State Univ, Ctr Hlth Sci, Dept Mol & Cellular Physiol, Shreveport, LA 71105 USA
[4] Baylor Coll Med, Dept Med, Houston, TX 77030 USA
[5] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA
[6] Houston VA Med Ctr, Pulm & Crit Care Sect, Houston, TX 77030 USA
关键词
microvascular permeability; endothelial barrier; protein diffusion; reperfusion injury; nitric oxide;
D O I
10.1038/sj.mn.7800135
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective: Fluorescently labeled albumin is used frequently as a tracer when monitoring microvascular permeability. Several fluorescent dyes are available for labeling protein, including fluorescein isothiocyanate (FITC) and Texas Red (TR). Because differences in leakage of dye-labeled proteins have been reported., the objective of the present study was to compare the accumulation of these two tracers in interstitium and lymph after the inflammatory event of ischemia-reperfusion. Methods: Anesthetized rats were injected intravenously with FITC-labeled albumin (FITC-alb) and TR-labeled albumin (TR-alb) before 30 minutes of mesenteric ischemia. Because the tracers leaked out of the microcirculation after reperfusion. accumulation in the surrounding buffer-superfused tissue. and in separate experiments. accumulation in lymph vessels, was defined as the ratio of tissue-to-plasma and lymph-to-plasma fluorescence. Results: Reperfusion induced a significant increase in tissue-to-plasma fluorescence of FITC-alb, however. no increase was observed for TR-alb. In contrast, lymph-to-plasma fluorescence of TR-alb tended to be greater than FITC-alb. Reperfusion-induced increases in tissue-to-plasma fluorescence of TR-alb occurred only when the superfusate was replaced with mineral oil. in which case tissue-to-plasma TR-alb fluorescence tended to be greater than FITC-alb. Conclusions: Measurement of fluorescently labeled albumin leak-age from mesenteric venules depends on the dye used to label the albumin and requires an assessment of losses from the extravascular measuring region.
引用
收藏
页码:177 / 187
页数:11
相关论文
共 44 条
[1]  
ADAMS DA, 1994, ONLINE J KNOWL SYN N, V1, P1
[2]  
Barber B. J., 1994, Microcirculation (New York), V1, P101, DOI 10.3109/10739689409148265
[3]   CHANGES IN RAT MESENTERY INTERSTITIAL MATRIX DUE TO SUPERFUSATE [J].
BARBER, BJ ;
BABBITT, RA ;
DUTTA, S ;
PARAMESWARAN, S .
AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 265 (03) :H852-H856
[4]  
Bingaman S, 1999, FASEB J, V13, pA4
[5]  
Carden DL, 2000, J PATHOL, V190, P255, DOI 10.1002/(SICI)1096-9896(200002)190:3<255::AID-PATH526>3.0.CO
[6]  
2-6
[7]  
Casley-Smith J R, 1979, Bibl Anat, P36
[8]  
CHRISTOPHER TA, 1995, J PHARMACOL EXP THER, V273, P64
[9]   Neutrophil priming state predicts capillary leak after gut ischemia in rats [J].
Conner, WC ;
Gallagher, CM ;
Miner, TJ ;
Tavaf-Motamen, H ;
Wolcott, KM ;
Shea-Donohue, T .
JOURNAL OF SURGICAL RESEARCH, 1999, 84 (01) :24-30
[10]   FUNCTIONAL CORONARY MICROVASCULAR INJURY EVIDENT AS INCREASED PERMEABILITY DUE TO BRIEF ISCHEMIA AND REPERFUSION [J].
DAUBER, IM ;
VANBENTHUYSEN, KM ;
MCMURTRY, IF ;
WHEELER, GS ;
LESNEFSKY, EJ ;
HORWITZ, LD ;
WEIL, JV .
CIRCULATION RESEARCH, 1990, 66 (04) :986-998