Effects of histone acetylation on transcriptional regulation of manganese superoxide dismutase gene

被引：37

作者：

Maehara, K ^{[1
]}

Uekawa, N ^{[1
]}

Isobe, KI ^{[1
]}

机构：

[1] Natl Inst Longev Sci, Dept Basic Gerontol, Aichi 4748522, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 295卷 / 01期

关键词：

manganese superoxide dismutase; histone acetylation; transcription; histone deacetylase-1; Sp1; Egr-1;

D O I：

10.1016/S0006-291X(02)00646-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To better understand the link between chromatin modification and manganese superoxide dismutase (Mn-SOD) gene expression, we have investigated the level of historic acetylation at Mn-SOD proximal promoter. TSA induced the expression of Mn-SOD mRNA and its transcriptional activity in C2C12 cells. Spl binding sites in the proximal promoter region of Mn-SOD were transcriptionally responsive to TSA by transfection studies. We have detected a localized acetylation of histories H3 and H4, in vivo occupation by Spl, early growth responsive-1 (Egr-1), and histone deacetylase-1 (HDAC1) in the proximal promoter region of MnSOD gene using chromatin immunoprecipitation assays. Our findings indicate that Mn-SOD gene expression is repressed by Spl-HDAC1 complex. This repression is released by a localized historic acetylation and at least in parts a displacement by Egr-1 in response to TSA. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：187 / 192

页数：6

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