In vivo molecular probing of cellular compartments with gold nanoparticles and nanoaggregates

被引：415

作者：

Kneipp, Janina

Kneipp, Harald

McLaughlin, Margaret

Brown, Dennis

Kneipp, Katrin

机构：

[1] Harvard Univ, Sch Med, Wellman Ctr Photomed, Boston, MA 02114 USA

[2] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Renal Unit, Boston, MA 02114 USA

来源：

NANO LETTERS | 2006年 / 6卷 / 10期

关键词：

D O I：

10.1021/nl061517x

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Surface-enhanced Raman (SERS) signatures were measured from single living cells at different times after the uptake of gold nanoparticles. The spectra are indicative of chemical changes in the environment of the nanostructures over time. The increase of the SERS signal strength and parallel TEM studies indicate the formation of nanoaggregates providing optimum SERS enhancement for ultrasensitive probing inside the endosomal compartment. The results have implications for medical and biotechnology applications of SERS nanosensors in cells.

引用

收藏

页码：2225 / 2231

页数：7

相关论文

共 52 条

[1] ANOMALOUSLY INTENSE RAMAN-SPECTRA OF PYRIDINE AT A SILVER ELECTRODE [J].

ALBRECHT, MG ;

CREIGHTON, JA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1977, 99 (15) :5215-5217

[2] A proteomic analysis of lysosomal integral membrane proteins reveals the diverse composition of the organelle [J].

Bagshaw, RD ;

Mahuran, DJ ;

Callahan, JW .

MOLECULAR & CELLULAR PROTEOMICS, 2005, 4 (02) :133-143

[3]

Blagojevic B., 2005, REV SCI INSTRUM, V76, DOI DOI 10.1063/1.1988227

[4]

Bright NA, 1997, J CELL SCI, V110, P2027

[5] Nanoparticles with Raman spectroscopic fingerprints for DNA and RNA detection [J].

Cao, YWC ;

Jin, RC ;

Mirkin, CA .

SCIENCE, 2002, 297 (5586) :1536-1540

[6] SURFACE-ENHANCED RAMAN-SCATTERING OF ADENOSINE-TRIPHOSPHATE MOLECULES [J].

CHEN, TT ;

KUO, CS ;

CHOU, YC ;

LIANG, NT .

LANGMUIR, 1989, 5 (04) :887-891

[7] Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells [J].

Chithrani, BD ;

Ghazani, AA ;

Chan, WCW .

NANO LETTERS, 2006, 6 (04) :662-668

[8] Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity [J].

Connor, EE ;

Mwamuka, J ;

Gole, A ;

Murphy, CJ ;

Wyatt, MD .

SMALL, 2005, 1 (03) :325-327

[9] Virulent Mycobacterium fortuitum restricts NO production by a gamma interferon-activated J774 cell line and phagosome-lysosome fusion [J].

da Silva, TRM ;

de Freitas, JR ;

Silva, QC ;

Figueira, CP ;

Roxo, E ;

Leao, SC ;

de Freitas, LAR ;

Veras, PST .

INFECTION AND IMMUNITY, 2002, 70 (10) :5628-5634

[10] Tat peptide as an efficient molecule to translocate gold nanoparticles into the cell nucleus [J].

de la Fuente, JM ;

Berry, CC .

BIOCONJUGATE CHEMISTRY, 2005, 16 (05) :1176-1180

← 1 2 3 4 5 6 →