Time-resolved total internal-reflection fluorometry of 1-pyrene sulfonate anion at a liquid/liquid interface

The fluorescence spectra and relevant dynamics of 1-pyrene sulfonate anion (PSA) at a water/1,2-dichloroethane (DCE) interface were studied by nanosecond time-resolved total internal reflection fluorometry. Both the fluorescence of PSA at a water/DCE interface and that in the water phase were observed under total internal-reflection conditions; also, the fluorescence characteristics at the interface could be separated from the observed spectrum on the basis of analyses of the fluorescence dynamics. The results are discussed in terms of the adsorption and dimer formation of PSA at the interface.