AIM: To investigate the hepatocytic differentiation ofmesenchymal stem cells (MSCs) in co-cultures with fetal liver cells (FLC) and the possibility to expand differentiated hepatocytic cells. METHODS: MSCs were marked with green fluorescent protein (GFP) by retroviral gene transduction. Clonal marked MSCs were either cultured under liver stimulating conditions using fibronectin-coated culture dishes and medium supplemented with stem cell factor (SCF), hepatocyte growth factor (HGF), epidermal growth factor (EGF), and fibroblast growth factor 4 (FGF-4) alone, or in presence of freshly isolated FLC. Cells in co-cultures were harvested, and GFP+ or GFP- cells were separated using fluorescence activated cell sorting. Reverse transcription-polymerase chain reaction (RT-PCR) for the liver specific markers cytokeratin-18 (CK-18), albumin, and alpha-fetoprotein (AFP) was performed in different cell populations. RESULTS: Under the specified culture conditions, rat MSCs co-cultured with FLC expressed albumin, CK-18, and AFP-RNA over two weeks. At A 3, MSCs lost hepatocytic gene expression, probably due to overgrowth of the cocultured FLC. FLC also showed a stable liver specific gene expression in the co-cultures and a very high growth potential. CONCLUSION: The rat MSCs from bone marrow can differentiate hepatocytic cells in the presence of FLC in vitro and the presence of MSCs in co-cultures also provides a beneficial environment for expansion and differentiation of FLC. (c) 2006 The WIG Press. All rights reserved.
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Avital L, 2001, BIOCHEM BIOPH RES CO, V288, P156
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Xi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Xi An Jiao Tong Univ, Dept Biol, Sch Med, Xian 710061, Shaanxi Provinc, Peoples R ChinaXi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Kang, Xin-Qin
Zang, Wei-Jin
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Xi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Xi An Jiao Tong Univ, Key Lab Biomed Informat Engn, Minist Educ, Sch Life Sci & Technol, Xian 710061, Shaanxi Provinc, Peoples R ChinaXi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Zang, Wei-Jin
Song, Tu-Sheng
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Xi An Jiao Tong Univ, Dept Biol, Sch Med, Xian 710061, Shaanxi Provinc, Peoples R ChinaXi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Song, Tu-Sheng
Xu, Xiao-Li
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Xi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R ChinaXi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Xu, Xiao-Li
Yu, Xiao-Jiang
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Xi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R ChinaXi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Yu, Xiao-Jiang
Li, Dong-Ling
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Xi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R ChinaXi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Li, Dong-Ling
Meng, Ke-Wei
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Xi An Jiao Tong Univ, Dept Hepatobiliary Surg, Hosp 1, Xian 710061, Shaanxi Provinc, Peoples R ChinaXi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Meng, Ke-Wei
Wu, Sheng-Li
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Xi An Jiao Tong Univ, Dept Hepatobiliary Surg, Hosp 1, Xian 710061, Shaanxi Provinc, Peoples R ChinaXi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Wu, Sheng-Li
Zhao, Zhi-Ying
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Xi An Jiao Tong Univ, Dept Anat, Sch Med, Xian 710061, Shaanxi Provinc, Peoples R ChinaXi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
机构:
Xi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Xi An Jiao Tong Univ, Dept Biol, Sch Med, Xian 710061, Shaanxi Provinc, Peoples R ChinaXi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Kang, Xin-Qin
Zang, Wei-Jin
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Xi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Xi An Jiao Tong Univ, Key Lab Biomed Informat Engn, Minist Educ, Sch Life Sci & Technol, Xian 710061, Shaanxi Provinc, Peoples R ChinaXi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Zang, Wei-Jin
Song, Tu-Sheng
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Xi An Jiao Tong Univ, Dept Biol, Sch Med, Xian 710061, Shaanxi Provinc, Peoples R ChinaXi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Song, Tu-Sheng
Xu, Xiao-Li
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Xi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R ChinaXi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Xu, Xiao-Li
Yu, Xiao-Jiang
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Xi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R ChinaXi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Yu, Xiao-Jiang
Li, Dong-Ling
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Xi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R ChinaXi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Li, Dong-Ling
Meng, Ke-Wei
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Xi An Jiao Tong Univ, Dept Hepatobiliary Surg, Hosp 1, Xian 710061, Shaanxi Provinc, Peoples R ChinaXi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Meng, Ke-Wei
Wu, Sheng-Li
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Xi An Jiao Tong Univ, Dept Hepatobiliary Surg, Hosp 1, Xian 710061, Shaanxi Provinc, Peoples R ChinaXi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China
Wu, Sheng-Li
Zhao, Zhi-Ying
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h-index: 0
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Xi An Jiao Tong Univ, Dept Anat, Sch Med, Xian 710061, Shaanxi Provinc, Peoples R ChinaXi An Jiao Tong Univ, Div Cardiovasc Physiol & Pharmacol, Sch Med, Xian 710061, Shaanxi Prov, Peoples R China