Preparation of alginate/galactosylated chitosan scaffold for hepatocyte attachment

Galactose-carrying lactobionic acid was covalently coupled with chitosan for determining hepatocyte specificity. Galactosylated chitosan (GC) was reacted with Ca-alginate (ALG) get through the electrostatic interaction of carboxylic groups of alginate with amine groups of GC. Highly porous, three-dimensional sponge composed of ALG and GC was prepared to provide specific hepatocyte recognition signals and enhance the mechanical property of the ALG sponge. Observation of the sponge through scanning electron microscopy revealed that sponge was a highly porous microstructure with interconnected pores. Porosity and pore size of the sponge were greatly dependent on the content and molecular weight of GC, and freezing temperature. The mechanical property of the ALG/GC sponge was enhanced with an increase of the GC content. Spheroid formation and viability of hepatocytes of the ALG/GC sponge were higher than those of the ALG one. (C) 2002 Elsevier Science Ltd. All rights reserved.