Effect of gender on endothelium-dependent dilation to bradykinin in human adipose microvessels

被引:20
作者
Sato, A
Miura, H
Liu, YP
Somberg, LB
Otterson, MF
Demeure, MJ
Schulte, WJ
Eberhardt, LM
Loberiza, FR
Sakuma, I
Gutterman, DD
机构
[1] Med Coll Wisconsin, Ctr Cardiovasc, Dept Internal Med, Milwaukee, WI 53226 USA
[2] Med Coll Wisconsin, Ctr Cardiovasc, Dept Surg, Milwaukee, WI 53226 USA
[3] Med Coll Wisconsin, Vet Adm Med Ctr, Milwaukee, WI 53226 USA
[4] Hokkaido Univ, Sch Med, Dept Cardiovasc Med, Sapporo, Hokkaido 0608638, Japan
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 2002年 / 283卷 / 03期
关键词
gender; bradykinin; human vessel; endothelium;
D O I
10.1152/ajpheart.00160.2002
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We examined the influence of gender and climacteric status, two coronary risk factors, on bradykinin (BK)-induced dilation in adipose arterioles from men and women of different ages [premenopausal women (Pre-W), postmenopausal women (Post-W), and similar aged men (Y-M and O-M), respectively]. We examined the responses from both omental (more closely associated with coronary disease) and subcutaneous fat. Tissues were obtained at surgery and cannulated (60 mmHg) for measurement of internal diameter. In vessels from omental tissue, dilation to BK was more sensitive in Pre-W than other groups, whereas in vessels from subcutaneous tissue, sensitivity to BK was greater in both Pre-W and Post-W compared with Y-M and O-M. Maximal dilation was similar among groups. Indomethacin (Indo; 10(-5) M) alone had no effect on dilation to BK in any groups, but Indo and N-omega-nitro-L-arginine methyl ester (L-NAME; 10(-4) M) reduced dilation to BK in Pre-W more than in Y-M. L-NAME increased dilation to BK in subcutaneous fat from Y-M but had no effect in Post-W and O-M. Indo- and L-NAME- resistant dilation in all vessels was markedly reduced by 30 mM KCl. There was no difference in sodium nitroprusside-induced dilation among groups. We conclude that gender and climacteric state contribute to mechanisms of microvascular regulation in humans. Functional vascular differences in visceral and subcutaneous fat may underlie the proposed differential influence of these tissues on cardiovascular risk.
引用
收藏
页码:H845 / H852
页数:8
相关论文
共 32 条
[1]   A nitric oxide-mediated mechanism regulates lipolysis in human adipose tissue in vivo [J].
Andersson, K ;
Gaudiot, N ;
Ribiere, C ;
Elizalde, M ;
Giudicelli, Y ;
Arner, P .
BRITISH JOURNAL OF PHARMACOLOGY, 1999, 126 (07) :1639-1645
[2]   Nitric oxide attenuates the release of endothelium-derived hyperpolarizing factor [J].
Bauersachs, J ;
Popp, R ;
Hecker, M ;
Sauer, E ;
Fleming, I ;
Busse, R .
CIRCULATION, 1996, 94 (12) :3341-3347
[3]   Gender differences in the generation of superoxide anions in the rat aorta [J].
Brandes, RP ;
Mugge, A .
LIFE SCIENCES, 1997, 60 (06) :391-396
[4]   Gender and nitric oxide-mediated vasodilation in humans [J].
Dietz, NM .
LUPUS, 1999, 8 (05) :402-408
[5]   Role of estrogen in modulating EDHF-mediated dilations in the female rat middle cerebral artery [J].
Golding, EM ;
Kepler, TE .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2001, 280 (06) :H2417-H2423
[6]   POSTMENOPAUSAL USE OF ESTROGEN AND OCCLUSION OF CORONARY-ARTERIES [J].
GRUCHOW, HW ;
ANDERSON, AJ ;
BARBORIAK, JJ ;
SOBOCINSKI, KA .
AMERICAN HEART JOURNAL, 1988, 115 (05) :954-963
[7]   Gender difference in myogenic tone of rat arterioles is due to estrogen-induced, enhanced release of NO [J].
Huang, A ;
Sun, D ;
Koller, A ;
Kaley, G .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 1997, 272 (04) :H1804-H1809
[8]   Gender difference in flow-induced dilation and regulation of shear stress: role of estrogen and nitric oxide [J].
Huang, A ;
Sun, D ;
Koller, A ;
Kaley, G .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 1998, 275 (05) :R1571-R1577
[9]   Temporal effects of 17β-estradiol on caveolin-1 mRNA and protein in bovine aortic endothelial cells [J].
Jayachandran, M ;
Hayashi, T ;
Sumi, D ;
Iguchi, A ;
Miller, VM .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2001, 281 (03) :H1327-H1333
[10]   Endothelial nitric oxide synthase is a site of superoxide synthesis in endothelial cells treated with glyceryl trinitrate [J].
Kaesemeyer, WH ;
Ogonowski, AA ;
Jin, LM ;
Caldwell, RB ;
Caldwell, RW .
BRITISH JOURNAL OF PHARMACOLOGY, 2000, 131 (05) :1019-1023