Evaluation of methods for molecular typing and identification of members of the genus Brevibacterium and other related species

被引:10
作者
Alves, A [1 ]
Santos, O [1 ]
Henriques, I [1 ]
Correia, A [1 ]
机构
[1] Univ Aveiro, Ctr Biol Celular, Dept Biol, P-3810193 Aveiro, Portugal
关键词
Brevibacterium; amplified ribosomal DNA restriction analysis; repetitive-sequence-based polymerase chain reaction; ribotyping; Brevibacterium linens;
D O I
10.1111/j.1574-6968.2002.tb11307.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The genus Brevibacterium includes pleomorphic Gram-positive bacteria with a high mol% G+C content. Species in the genus are difficult to identify by classical methods. The discriminatory power of DNA-based methods is assessed. Strains representing the four well established Brevibacterium species, and other related bacteria, were compared by amplified ribosomal DNA restriction analysis (ARDRA), repetitive-sequence-based PCR (rep-PCR) and ribotyping. Fingerprinting by rep-PCR and ribotyping provided complex genomic profiles with the highest discriminatory potential for molecular typing at the strain level, whereas ARDRA showed differentiation from the genus to the species levels. A high degree of heterogeneity within the genus Brevibacterium is apparent, thus indicating that the taxonomy of the genus should be further studied. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:205 / 211
页数:7
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