Survivin expression in rat testis is upregulated by stem-cell factor

被引:26
作者
Wang, YY
Suominen, JS
Hakovirta, H
Parvinen, M
Martinand-Mari, C
Toppari, J
Robbins, I
机构
[1] Inst Genet Mol, UMR 5124, F-34293 Montpellier 5, France
[2] Turku Univ, Dept Physiol & Pediat, Turku 20520, Finland
[3] Turku Univ, Dept Anat, Turku 20520, Finland
[4] Turku Univ, Dept Surg, Turku 20520, Finland
基金
芬兰科学院;
关键词
BIRC-5; survivin; testis; meiosis; spermatocyte;
D O I
10.1016/j.mce.2003.11.028
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The inhibitor of apoptosis protein BIRC-5/survivin plays roles in both apoptosis and the regulation of chromosome-segregation/cytokinesis during mitosis. As the population dynamics of male germ cells are regulated by both proliferation (mitosis and meiosis) and apoptotic culling, we hypothesized that BIRC-5/survivin could be central to the regulation of spermatogenesis. We have analyzed BIRC-5/survivin expression throughout the seminiferous epithelial cycle of the rat. BIRC-5/survivin RNA and protein exhibit rhythms of expression throughout the seminiferous epithelial cycle. The highest levels of expression were found, by immunohistochemistry and in situ hybridization, to occur during the long first meiotic prophase of spermatocytes. Cytoplasmic abundance declined at metaphase and reappeared at anaphase. Some BIRC-5/survivin expression was also found to occur in interstitial Leydig cells. BIRC-5/survivin protein levels were up-regulated in vitro by the paracrine, Stem-Cell Factor, that is known to regulate both proliferation and apoptosis of germ cells and Leydig cells. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:165 / 174
页数:10
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