Carbohydrate release from picomole quantities of glycoprotein and characterisation of glycans by high-performance liquid chromatography and mass spectrometry

被引：17

作者：

Charlwood, J ^{[1
]}

Birrell, H ^{[1
]}

Camilleri, P ^{[1
]}

机构：

[1] SmithKline Beecham Pharmaceut, Harlow CM19 5AW, Essex, England

来源：

JOURNAL OF CHROMATOGRAPHY B | 1999年 / 734卷 / 01期

关键词：

glycoproteins; glycans; carbohydrates; immunoglobulins;

D O I：

10.1016/S0378-4347(99)00330-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Samples of 5 to 20 mu g of human IgG were subjected to dithiothreitol treatment to reduce disulphide bridges, followed by tryptic digestion. Glycans released from the tryptic peptide mixture by PNGase F digestion were then derivatised with 2-aminoacridone. Labelled oligosaccharides were separated by normal-phase high-performance liquid chromatography and individual components were collected for matrix-assisted laser desorption ionization time-of-flight and electrospray mass spectrometric analysis. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：169 / 174

页数：6

共 9 条

[1] A dual-detection strategy in the chromatographic analysis of 2-aminoacridone-derivatized oligosaccharides [J].

Birrell, H ;

Charlwood, J ;

Lynch, I ;

North, S ;

Camilleri, P .

ANALYTICAL CHEMISTRY, 1999, 71 (01) :102-108

[2] Efficient carbohydrate release, purification, and derivatization [J].

Charlwood, J ;

Birrell, H ;

Camilleri, P .

ANALYTICAL BIOCHEMISTRY, 1998, 262 (02) :197-200

[3]

Charlwood J, 1999, RAPID COMMUN MASS SP, V13, P107, DOI 10.1002/(SICI)1097-0231(19990130)13:2<107::AID-RCM463>3.0.CO

[4]

2-P

[5] HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY FOR CARBOHYDRATE ANALYSIS [J].

LEE, YC .

ANALYTICAL BIOCHEMISTRY, 1990, 189 (02) :151-162

[6] CAPILLARY ZONE ELECTROPHORESIS OF LINEAR AND BRANCHED OLIGOSACCHARIDES [J].

NASHABEH, W ;

ELRASSI, Z .

JOURNAL OF CHROMATOGRAPHY, 1992, 600 (02) :279-287

[7] A coordinated high-performance liquid chromatographic, capillary electrophoretic, and mass spectrometric approach for the analysis of oligosaccharide mixtures derivatized with 2-aminoacridone [J].

Okafo, G ;

Burrow, L ;

Carr, SA ;

Roberts, GD ;

Johnson, W ;

Camilleri, P .

ANALYTICAL CHEMISTRY, 1996, 68 (24) :4424-4430

[8] High performance liquid chromatographic analysis of complex N-linked glycans derivatized with 2-aminoacridone [J].

Okafo, G ;

Langridge, J ;

North, S ;

Organ, A ;

West, A ;

Morris, M ;

Camilleri, P .

ANALYTICAL CHEMISTRY, 1997, 69 (24) :4985-4993

[9] Simple differentiation between core-fucosylated and nonfucosylated glycans by capillary electrophoresis [J].

Okafo, GN ;

Burrow, LM ;

Neville, W ;

Truneh, A ;

Smith, RAG ;

Reff, M ;

Camilleri, P .

ANALYTICAL BIOCHEMISTRY, 1996, 240 (01) :68-74

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