Nitrosomonas europaea expresses a nitric oxide reductase during nitrification

被引：72

作者：

Beaumont, HJE ^{[1
]}

van Schooten, B ^{[1
]}

Lens, SI ^{[1
]}

Westerhoff, HV ^{[1
]}

van Spanning, RJM ^{[1
]}

机构：

[1] Free Univ Amsterdam, Biocentrum Amsterdam, Dept Mol Cell Physiol, NL-1081 HV Amsterdam, Netherlands

来源：

JOURNAL OF BACTERIOLOGY | 2004年 / 186卷 / 13期

关键词：

D O I：

10.1128/JB.186.13.4417-4421.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In this paper, we report the identification of a norCBQD gene cluster that encodes a functional nitric oxide reductase (Nor) in Nitrosomonas europaea. Disruption of the norB gene resulted in a strongly diminished nitric oxide (NO) consumption by cells and membrane protein fractions, which was restored by the introduction of an intact norCBQD gene cluster in trans. NorB-deficient cells produced amounts of nitrous oxide (N2O) equal to that of wild-type cells. NorCB-dependent activity was present during aerobic growth and was not affected by the inactivation of the putative fnr gene. The findings demonstrate the presence of an alternative site of N2O production in N. europaea.

引用

页码：4417 / 4421

页数：5

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