Quantification of perfume compounds in shampoo using solid-phase microextraction

Methods for the quantitative analysis of perfume compounds in shampoo were developed using SPME (solid-phase microextraction). The method development started with the extraction of the perfume compounds from water, and subsequently from shampoo aqueous dispersion. It was observed that the addition of salts decreased the extraction efficiencies with the concentration of shampoo in water as low as 0.1% in contrast to the effect of the salt in water. Step-by-step optimization to increase extraction efficiency and minimize matrix effect was investigated. Dilution of shampoo with water was found to be an efficient method for quantification. It decreased the viscosity of the shampoo and increased the concentration of free analytes. Largely diluted shampoos enabled an efficient SPME extraction and allowed the use of a unified calibration applicable to different types of shampoo. The exhaustive SPME extraction of small amounts of sample, e.g. 50 mu l, provided another fast quantitative method. This unprecedented work presents quantitative methods for the analysis of perfume compounds in shampoo. This research extends the knowledge of perception and reconstitution of perfume compounds in shampoo, and facilitates development of new detergent products. Copyright (c) 2006 John Wiley & Sons, Ltd.