Pharmacological Inhibition of Gal-3 in Mesenchymal Stem Cells Enhances Their Capacity to Promote Alternative Activation of Macrophages in Dextran Sulphate Sodium-Induced Colitis

被引：30

作者：

Markovic, Bojana Simovic ^{[1
]}

Nikolic, Aleksandar ^{[1
]}

Gazdic, Marina ^{[1
]}

Nurkovic, Jasmin ^{[2
]}

Djordjevic, Irena ^{[1
]}

Arsenijevic, Nebojsa ^{[1
]}

Stojkovic, Miodrag ^{[1
,3
]}

Lukic, Miodrag L. ^{[1
]}

Volarevic, Vladislav ^{[1
]}

机构：

[1] Univ Kragujevac, Fac Med Sci, Ctr Mol Med & Stem Cell Res, 69 Svetozar Markovic St, Kragujevac 34000, Serbia

[2] State Univ Novi Pazar, Dept Biomed Sci, Stem Cell Lab, Nn Vuk Karadzic St, Novi Pazar 36300, Serbia

[3] Spebo Med, 16 Norvezanska St, Leskovac 16000, Serbia

来源：

STEM CELLS INTERNATIONAL | 2016年 / 2016卷

关键词：

DENDRITIC CELLS; TARGETED DISRUPTION; STROMAL CELLS; GALECTIN-3; MICE; INFLAMMATION; PROLIFERATION; DISEASE; MODELS; INJURY;

D O I：

10.1155/2016/2640746

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

Transplantation of mesenchymal stem cells (MSCs) reduces the severity of dextran sulphate sodium-(DSS-) induced colitis. MSCs are able to secrete Galectin-3 (Gal-3), a protein known to affect proliferation, adhesion, and migration of immune cells. We investigate whether newly synthetized inhibitor of Gal-3 (Davanat) will affect production of Gal-3 in MSCs and enhance their potential to attenuate DSS-induced colitis. Pharmacological inhibition of Gal-3 in MSCs enhances their capacity to promote alternative activation of peritoneal macrophages in vitro and in vivo. Injection of MSCs cultured in the presence of Davanat increased concentration of IL-10 in sera of DSS-treated animals and markedly enhanced presence of alternatively activated and IL-10 producing macrophages in the colons of DSS-treated mice. Pharmacological inhibition of Gal-3 in MSCs significantly attenuates concentration of Gal-3 in sera of DSS-treated animals, indicating that MSCs produce Gal-3 in this disease. In conclusion, our findings indicate that Davanat could be used for improvement of MSC-mediated polarization towards immunosuppressive M2 phenotype of macrophages.

引用

页数：12

共 39 条

[1]

Colitis induced in mice with dextran sulfate sodium (DSS) is mediated by the NLRP3 inflammasome [J].