Auxofuran, a novel metabolite that stimulates the growth of fly agaric, is produced by the mycorrhiza helper bacterium Streptomyces strain AcH 505

被引:122
作者
Riedlinger, Julia
Schrey, Silvia D.
Tarkka, Mika T.
Hampp, Ruediger
Kapur, Manmohan
Fiedler, Hans-Peter
机构
[1] Univ Tubingen, Bot Inst, D-72076 Tubingen, Germany
[2] Univ Tubingen, Mikrobiol Inst, D-72076 Tubingen, Germany
[3] Univ Tubingen, Inst Organ Chem, D-72076 Tubingen, Germany
关键词
D O I
10.1128/AEM.72.5.3550-3557.2006
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The mycorrhiza helper bacterium Streptomyces strain AcH 505 improves mycelial growth of ectomycorrhizal fungi and formation of ectomycorrhizas between Amanita muscaria and spruce but suppresses the growth of plant-pathogenic fungi, suggesting that it produces both fungal growth-stimulating and -suppressing compounds. The dominant fungal-growth-promoting substance produced by strain AcH 505, auxoforan, was isolated, and its effect on the levels of gene expression of A. muscaria was investigated. Auxofuran and its synthetic analogue 7-dehydroxy-auxofuran were most effective at a concentration of 15 mu M, and application of these compounds led to increased lipid metabolism-related gene expression. Cocultivation of strain AcH 505 and A. muscaria stimulated auxofuran production by the streptomycete. The antifungal substances produced by strain AcH 505 were identified as the antibiotics WS-5995 B and C. WS-5995 B completely blocked mycelial growth at a concentration of 60 mu M and caused a cell stress-related gene expression response in A. muscaria. Characterization of these compounds provides the foundation for molecular analysis of the fungus-bacterium interaction in the ectomycorrhizal symbiosis between fly agaric and spruce.
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页码:3550 / 3557
页数:8
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