Improved base calling for the Illumina Genome Analyzer using machine learning strategies

被引：169

作者：

Kircher, Martin ^{[1
]}

Stenzel, Udo ^{[1
]}

Kelso, Janet ^{[1
]}

机构：

[1] Max Planck Inst Evolutionary Anthropol, Dept Evolutionary Genet, D-04103 Leipzig, Germany

来源：

GENOME BIOLOGY | 2009年 / 10卷 / 08期

关键词：

Quality Score; Additional Data File; Base Calling; Base Caller; Sequencing Chemistry;

D O I：

10.1186/gb-2009-10-8-r83

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Illumina Genome Analyzer generates millions of short sequencing reads. We present Ibis (Improved base identification system), an accurate, fast and easy-to-use base caller that significantly reduces the error rate and increases the output of usable reads. Ibis is faster and more robust with respect to chemistry and technology than other publicly available packages. Ibis is freely available under the GPL from http://bioinf.eva.mpg.de/Ibis/.

引用

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