Characterization of the immune response to canine parvovirus induced by vaccination with chimaeric plant viruses

被引:32
作者
Nicholas, BL [1 ]
Brennan, FR
Martinez-Torrecuadrada, JL
Casale, JI
Hamilton, WD
Wakelin, D
机构
[1] Univ Nottingham, Sch Biosci, Loughborough LE12 5RD, Leics, England
[2] Univ Nottingham, Sch Life & Envrionm Sci, Nottingham NG7 2RD, England
[3] Huntingdon Life Sci, Cambridge PE28 4HS, England
[4] INGENASA, Madrid 28037, Spain
[5] KCNIO, Program Biotecnol, Madrid 28029, Spain
[6] Proteom Ltd, Cambridge CB2 4AT, England
关键词
mucosal; immunity; virus;
D O I
10.1016/S0264-410X(02)00200-1
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
NIH mice were vaccinated subcutaneously or intranasally with chimaeric cow pea mosaic virus (CPMV) constructs expressing a 17-mer peptide sequence from canine parvovirus (CPV) as monomers or dimers on the small or large protein surface subunits. Responses to the chimaeric virus particles (CVPs) were compared with those of mice immunized with the native virus or with parvovirus peptide conjugated to keyhole limpet haemocyanin (KLH). The characteristics of the immune response to vaccination were examined by measuring serum and mucosal antibody responses in ELISA, in vitro antigen-induced spleen cell proliferation and cytokine responses. Mice made strong antibody responses to the native plant virus and peptide-specific responses to two of the four CVP constructs tested which were approximately 10-fold lower than responses to native plant virus. The immune response generated by the CVP constructs showed a marked TH1 bias, as determined by a predominantly IgG(2a) isotype peptide-specific antibody response and the release of IFN-gamma but not IL-4 or IL-5 from lymphocytes exposed to antigen in vitro. In comparison, parvovirus peptide conjugated to KLH generated an IgG(1)-biased (TH2) response. These data indicate that the presentation of peptides on viral particles could be used to bias the immune response in favor of a TH1 response. Anti-viral and anti-peptide IgA were detected in intestinal and bronchial lavage fluid of immunized mice, demonstrating that a mucosal immune response to CPV can be generated by systemic and mucosal immunization with CVP vaccines. Serum antibody from both subcutaneously-vaccinated and intranasally-vaccinated mice showed neutralizing activity against CPV in vitro. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:2727 / 2734
页数:8
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