Distinction between a transgenic and a conventional common bean genotype by 1H HR-MAS NMR

被引:28
作者
Choze, Rafael [1 ]
Alcantara, Glaucia B. [1 ]
Alves Filho, Elenilson de G. [1 ]
e Silva, Lorena Mara A. [1 ]
Faria, Josias C. [2 ]
Liao, Luciano M. [1 ]
机构
[1] Univ Fed Goias, Inst Quim, BR-74001970 Goiania, Go, Brazil
[2] Embrapa Arroz & Feijao, BR-75375000 Santo Antonio De Goias, Go, Brazil
关键词
Phaseolus vulgaris; Common beans; Transgenic; HR-MAS NMR; MAGNETIC-RESONANCE-SPECTROSCOPY; PHASEOLUS-VULGARIS; IDENTIFICATION;
D O I
10.1016/j.foodchem.2013.05.123
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
In this work, it is proposed a methodology which allows to differentiate a conventional and a specific transgenic common beans, grown in greenhouse or under field conditions, based on modifications in chemical composition using H-1 HR-MAS NMR. It is demonstrated that the influence of typical variables from field planting conditions had no significant influence on the ability to set apart transgenic from conventional. This methodology was corroborated by multivariate data analysis of the H-1 NMR and IR spectra. This study also points out the simplicity of using the HR-MAS NMR technique for food analyses. The measurement is highly simplified because it does not require any pretreatment of the sample apart from the addition of a small amount of D2O necessary to produce homogeneous dough and a field frequency lock. Moreover, due to the high concentration of the sample, measurement time in HR-MAS NMR is very short. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:2841 / 2847
页数:7
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