Identification of Treponema pallidum subspecies pallidum in a 200-year-old skeletal specimen

被引：83

作者：

Kolman, CJ

Centurion-Lara, A

Lukehart, SA

Owsley, DW

Tuross, N

机构：

[1] Smithsonian Ctr Mat Res & Educ, Suitland, MD 20746 USA

[2] Univ Washington, Dept Med, Seattle, WA USA

[3] Smithsonian Inst, Dept Anthropol, Washington, DC 20560 USA

来源：

JOURNAL OF INFECTIOUS DISEASES | 1999年 / 180卷 / 06期

关键词：

D O I：

10.1086/315151

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Treponema pallidum subsp, pallidum, the causative agent of venereal syphilis, was detected in a 200-year-old skeletal specimen from Easter Island. An initial diagnosis of treponemal infection was confirmed by extensive purification of immunoglobulin that reacted strongly with T. pallidum antigen, Extracted DNA exhibited a single-base polymorphism that distinguished T.p. subsp, pallidum from 4 other human and nonhuman treponemes, Extensive precautions against contamination of the subject matter with modern treponemal DNA were employed, including analysis of archaeological and modern specimens in 2 geographically separate laboratories. Molecular determination of historical disease scares by using skeletal material can significantly enhance our understanding of the pathology and spread of infectious diseases.

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页码：2060 / 2063

页数：4

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