A simple, low-cost staining method for rapid-throughput analysis of tumor spheroids

被引：11

作者：

Eckerdt, Frank ^{[1
,2
]}

Alvarez, Angel ^{[3
]}

Bell, Jonathan ^{[1
,2
]}

Arvanitis, Constadina ^{[4
,5
]}

Iqbal, Asneha ^{[1
,2
]}

Arslan, Ahmet D. ^{[1
,2
]}

Hu, Bo ^{[3
]}

Cheng, Shi-Yuan ^{[3
]}

Goldman, Stewart ^{[1
,2
,6
]}

Platanias, Leonidas C. ^{[1
,2
,7
]}

机构：

[1] Northwestern Univ, Feinberg Sch Med, Robert H Lurie Comprehens Canc Ctr, Chicago, IL 60611 USA

[2] Northwestern Univ, Feinberg Sch Med, Div Hematol Oncol, Chicago, IL 60611 USA

[3] Northwestern Univ, Feinberg Sch Med, Robert H Lurie Comprehens Canc Ctr, Dept Neurol,Northwestern Brain Tumor Inst, Chicago, IL 60611 USA

[4] Northwestern Univ, Feinberg Sch Med, Ctr Adv Microscopy, Chicago, IL 60611 USA

[5] Northwestern Univ, Feinberg Sch Med, Dept Pathol, Chicago, IL 60611 USA

[6] Ann & Robert H Lurie Childrens Hosp Chicago, Div Hematol & Oncol, Chicago, IL 60611 USA

[7] Jesse Brown VA Med Ctr, Dept Med, Chicago, IL USA

来源：

BIOTECHNIQUES | 2016年 / 60卷 / 01期

关键词：

neurospheres; tumor spheroids; cancer stem cell; glioblastoma; acridine orange; microscopy; CANCER; CELLS;

D O I：

10.2144/000114372

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Tumor spheroids are becoming an important tool for the investigation of cancer stem cell (CSC) function in tumors; thus, low-cost and high-throughput methods for drug screening of tumor spheroids are needed. Using neurospheres as non-adherent three-dimensional (3-D) cultures, we developed a simple, low-cost acridine orange (AO)-based method that allows for rapid analysis of live neurospheres by fluorescence microscopy in a 96-well format. This assay measures the cross-section area of a spheroid, which corresponds to cell viability. Our novel method allows rapid screening of a panel of anti-proliferative drugs to assess inhibitory effects on the growth of cancer stem cells in 3-D cultures.

引用

收藏

页码：43 / 46

页数：4

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