Absorption of α-ketoglutarate by the gastrointestinal tract of pigs

被引:24
作者
Buddington, RK [1 ]
Pajor, A
Buddington, KK
Pierzynowski, S
机构
[1] Mississippi State Univ, Dept Sci Biol, Mississippi State, MS 39762 USA
[2] Univ Texas, Dept Physiol & Biophys, Med Branch, Galveston, TX 77555 USA
[3] Gramineer Int AB, AN Ideon Beta, SE-223 Lund, Sweden
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY | 2004年 / 138卷 / 02期
关键词
absorption; alpha-ketoglutarate; colon; dicarboxylic acid; NaDC-1; small intestine; stomach; transporter;
D O I
10.1016/j.cbpb.2004.03.007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Only a small percentage of alpha-ketoglutarate (AKG) administered lumenally to pigs appears in the portal circulation. This has been attributed to mucosal metabolism, and possibly by limited absorption. Although transporters for di- and tricarboxylic acids, which includes the sodium-dependent transporter NaDC-1, have been detected in the small intestine, correlations with functional assays are lacking. Therefore, intact tissues from three regions of the small intestine, stomach, and colon of weaned pigs were used to measure rates of AKG absorption. Western analysis was used to detect NaDC-1 in the three regions of small intestine. Rates of AKG absorption were highest in the small intestine, lowest in the colon, and intermediate in the stomach. Immunoreactive NaDC-1 was detected in the small intestine and this coincided with a component of AKG absorption that was inhibited by AKG and succinate. In contrast, absorption of AKG was inhibitable by unlabeled AKG, but not succinate, in the stomach, and by neither in the colon. Feeding studies indicated that the amounts of AKG that might be included in practical diets for pigs would not (1) upregulate rates of AKG absorption or (2) exceed estimated capacities of the small intestine to absorb AKG. The present findings indicate that the efficacy of AKG as an alternative metabolic fuel for enterocytes to spare dietary amino acids is not limited by absorption. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:215 / 220
页数:6
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