Induction of human cytotoxic T lymphocytes by artificial antigen-presenting cells

被引:147
作者
Latouche, JB
Sadelain, M
机构
[1] Mem Sloan Kettering Canc Ctr, Dept Human Genet, New York, NY 10021 USA
[2] Mem Sloan Kettering Canc Ctr, Program Immunol, New York, NY 10021 USA
关键词
adoptive cell therapy; CD8(+) T cell; costimulation; dendritic cells; immunotherapy; retrovirus-mediated gene transfer;
D O I
10.1038/74455
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) is a promising therapeutic approach for a number of diseases. To overcome the difficulty in generating specific CTLs, we established stable artificial antigen-presenting cells (AAPCs) that can be used to stimulate T cells of any patient of a given human leukocyte antigen (HLA) type. Mouse fibroblasts were retrovirally transduced with a single HLA-peptide complex along with the human accessory molecules B7.1, ICAM-1, and LFA-3. These AAPCs consistently elicit strong stimulation and expansion of HLA-restricted CTLs. Owing to the high efficiency of retrovirus-mediated gene transfer, stable AAPCs can be readily engineered for any HLA molecule and any specific peptide.
引用
收藏
页码:405 / 409
页数:5
相关论文
共 46 条
  • [1] ENDOGENOUSLY SYNTHESIZED PEPTIDE WITH AN ENDOPLASMIC-RETICULUM SIGNAL SEQUENCE SENSITIZES ANTIGEN PROCESSING MUTANT-CELLS TO CLASS-I-RESTRICTED CELL-MEDIATED LYSIS
    ANDERSON, K
    CRESSWELL, P
    GAMMON, M
    HERMES, J
    WILLIAMSON, A
    ZWEERINK, H
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1991, 174 (02) : 489 - 492
  • [2] Dendritic cells and the control of immunity
    Banchereau, J
    Steinman, RM
    [J]. NATURE, 1998, 392 (6673) : 245 - 252
  • [3] BEDNAREK MA, 1991, J IMMUNOL, V147, P4047
  • [4] Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood
    Bender, A
    Sapp, M
    Schuler, G
    Steinman, RM
    Bhardwaj, N
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1996, 196 (02) : 121 - 135
  • [5] INFLUENZA VIRUS-INFECTED DENDRITIC CELLS STIMULATE STRONG PROLIFERATIVE AND CYTOLYTIC RESPONSES FROM HUMAN CD8+ T-CELLS
    BHARDWAJ, N
    BENDER, A
    GONZALEZ, N
    BUI, LK
    GARRETT, MC
    STEINMAN, RM
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1994, 94 (02) : 797 - 807
  • [6] Gene and cell transfer for specific immunotherapy
    Brenner, MK
    Heslop, HE
    Rooney, CM
    [J]. VOX SANGUINIS, 1998, 74 : 87 - 90
  • [7] CALCULATION OF LYTIC UNITS FOR THE EXPRESSION OF CELL-MEDIATED CYTOTOXICITY
    BRYANT, J
    DAY, R
    WHITESIDE, TL
    HERBERMAN, RB
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1992, 146 (01) : 91 - 103
  • [8] Serological analysis of Melan-A(MART-1), a melanocyte-specific protein homogeneously expressed in human melanomas
    Chen, YT
    Stockert, E
    Jungbluth, A
    Tsang, SL
    Coplan, KA
    Scanlan, MJ
    Old, LJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (12) : 5915 - 5919
  • [9] TOPOLOGY AND AFFINITY OF T-CELL RECEPTOR MEDIATED RECOGNITION OF PEPTIDE MHC COMPLEXES
    DAVIS, MM
    CHIEN, YH
    [J]. CURRENT OPINION IN IMMUNOLOGY, 1993, 5 (01) : 45 - 49
  • [10] Deeths MJ, 1999, EUR J IMMUNOL, V29, P45, DOI 10.1002/(SICI)1521-4141(199901)29:01<45::AID-IMMU45>3.0.CO