Human napsin A:: expression, immunochemical detection, and tissue localization

被引：51

作者：

Schauer-Vukasinovic, V ^{[1
]}

Bur, D ^{[1
]}

Kling, D ^{[1
]}

Grüninger, F ^{[1
]}

Giller, T ^{[1
]}

机构：

[1] F Hoffmann La Roche & Co Ltd, Div Pharma, CH-4070 Basel, Switzerland

来源：

FEBS LETTERS | 1999年 / 462卷 / 1-2期

关键词：

human napsin A; expression; napsin A antibody; immunohistochemistry; human kidney; human lung;

D O I：

10.1016/S0014-5793(99)01458-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel aspartic proteinase, called napsin, has recently been found in human and mouse. Due to high similarity with cathepsin D a structural model of human napsin A could be built. Based on this model a potential epitope SFYLNRDPEEPDGGE has been identified, which was used to immunize rabbits. The resulting antibody was employed in monitoring the expression of recombinant human napsin A in HEK293 cell line. Western blot analysis confirmed the specificity of the antibody and showed that human napsin A is expressed as a single chain protein with the molecular weight of approximately 38 kDa, Immunohistochemical studies revealed high expression levels of napsin A in human kidney and lung but low expression in spleen. (C) 1999 Federation of European Biochemical Societies.

引用

页码：135 / 139

页数：5

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