Estimating parasitism levels in Lygus spp. (Hemiptera: Miridae) field populations using standard and molecular techniques

被引:31
作者
Ashfaq, M [1 ]
Braun, L [1 ]
Hegedus, D [1 ]
Erlandson, M [1 ]
机构
[1] Agr & Agri Food Canada, Saskatoon Res Ctr, Saskatoon, SK S7N 0X2, Canada
关键词
Peristenus; parasitism level; Lygus spp; PCR;
D O I
10.1080/09583150410001683592
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Host dissection, host rearing and PCR analysis were used to estimate parasitism level and identify the parasitoid complex in Lygus spp. populations in alfalfa. Parasitism level estimates by PCR were higher than by other methods. The PCR results from all samples tested with available primers were consistent with Peristenus pallipes , although the parasitoid was identified as Peristenus near mellipes by morphological characters.
引用
收藏
页码:731 / 735
页数:5
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