Liquid chromatographic analysis of dihydrostreptomycin sulfate

被引：15

作者：

Adams, E ^{[1
]}

Roets, E ^{[1
]}

Hoogmartens, J ^{[1
]}

机构：

[1] Catholic Univ Louvain, Fac Farmaceut Wetenschappen, Lab Farmaceut Chem Anal Geneesmiddelen, B-3000 Louvain, Belgium

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1999年 / 21卷 / 04期

关键词：

dihydrostreptomycin; liquid chromatography;

D O I：

10.1016/S0731-7085(99)00206-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The analysis of dihydrostreptomycin sulfate using a column packed with base deactivated reversed phase silica gel and ultraviolet detection at 205 nm is described. The mobile phase consists of an aqueous solution containing 4 g/l of sodium sulfate, 1.5 g/l of sodium octanesulfonate, 100 ml/l of acetonitrile and 50 ml/l of a 0.2-M phosphate buffer at pH 3.0. The method allows separation of streptidine, dihydrostreptomycin B, streptomycin, dihydrostreptomycin and deoxydihydrostreptomycin, as well as some other components which were not identified. The total time of analysis is 55 min. The effects of the different chromatographic parameters on the separation were also investigated. A number of commercial samples were analyzed using this method. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：715 / 722

页数：8

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