SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs

被引：357

作者：

Shaikh, Tanvir R. ^{[1
]}

Gao, Haixiao ^{[1
]}

Baxter, William T. ^{[1
]}

Asturias, Francisco J. ^{[2
]}

Boisset, Nicolas ^{[3
]}

Leith, Ardean ^{[1
]}

Frank, Joachim ^{[1
,4
,5
]}

机构：

[1] Wadsworth Ctr, Albany, NY 12201 USA

[2] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

[3] Univ P&M Curie, Dept Biol Struct, IMPMC, UMR CNRS 7590, F-75015 Paris, France

[4] Howard Hughes Med Inst, Dept Biochem & Mol Biophys, New York, NY 10032 USA

[5] Columbia Univ, Dept Biol Sci, New York, NY 10027 USA

来源：

NATURE PROTOCOLS | 2008年 / 3卷 / 12期

关键词：

D O I：

10.1038/nprot.2008.156

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This protocol describes the reconstruction of biological molecules from the electron micrographs of single particles. Computation here is performed using the image-processing software SPIDER and can be managed using a graphical user interface, termed the SPIDER Reconstruction Engine. Two approaches are described to obtain an initial reconstruction: random-conical tilt and common lines. Once an existing model is available, reference-based alignment can be used, a procedure that can be iterated. Also described is supervised classification, a method to look for homogeneous subsets when multiple known conformations of the molecule may coexist.

引用

页码：1941 / 1974

页数：34

共 36 条

[1] Special issue: Advances in computational image processing for microscopy [J].