Transformation of Acinetobacter sp strain BD413(pFG4ΔnptII) with transgenic plant DNA in soil microcosms and effects of kanamycin on selection of transformants

被引:74
作者
Nielsen, KM
van Elsas, JD
Smalla, K
机构
[1] Res Inst Plant Protect, IPO, DLO, NL-6700 GW Wageningen, Netherlands
[2] Norwegian Univ Sci & Technol, Unigen, N-7491 Trondheim, Norway
[3] Norwegian Univ Sci & Technol, Dept Bot, N-7491 Trondheim, Norway
[4] Fed Biol Res Ctr Agr & Forestry, BBA, Inst Plant Virol Microbiol & Biosafety, D-38104 Braunschweig, Germany
关键词
D O I
10.1128/AEM.66.3.1237-1242.2000
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Here we show that horizontal transfer of DNA, extracted from transgenic sugar beets, to bacteria, based on homologous recombination, can occur in soil. Restoration of a 317-bp-deleted nptII gene in Acinetobacter sp. strain BD413(pFG4) cells incubated in sterile soil microcosms was detected after addition of nutrients and transgenic plant DNA encoding a functional nptII gene conferring bacterial kanamycin resistance. Selective effects of the addition of kanamycin on the population dynamics of Acinetobacter sp. cells in soil were found, and high concentrations of kanamycin reduced the CFU of Acinetobacter sp. cells from 10(9) CFU/g of soil to below detection. In contrast to a chromosomal nptII-encoded kanamycin resistance, the pFG4-generated resistance was found to be unstable over a 31-day incubation period in vitro.
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页码:1237 / 1242
页数:6
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