Direct determination of single-to-double stranded DNA ratio in solution applying time-resolved fluorescence measurements of dye-DNA complexes

被引：30

作者：

Cosa, G

Focsaneanu, KS

McLean, JRN

Scaiano, JC ^{[1
]}

机构：

[1] Univ Ottawa, Dept Chem, Ottawa, ON K1N 6N5, Canada

[2] Hlth Canada, Radiat Protect Branch, Ottawa, ON K1A 1C1, Canada

来源：

CHEMICAL COMMUNICATIONS | 2000年 / 08期

关键词：

D O I：

10.1039/b000473l

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report the fluorescence lifetimes of the DNA-stain dye PicoGreen and discuss the difference exhibited in the upon binding to single-stranded vs. double-stranded DNA; we here developed a direct method for determining single-to-double stranded DNA ratios in solution by measurement of the pre-exponential factors in the fluorescence decay traces of dye-DNA complexes.

引用

页码：689 / 690

页数：2

共 17 条

[1]

ARNDTJOVIN DJ, 1989, METHOD CELL BIOL, V30, P417

[2]

Böcker W, 1999, CYTOMETRY, V35, P134

[3] OPTICAL AND PHOTOPHYSICAL PROPERTIES OF THE OXAZOLE YELLOW DNA PROBES YO AND YOYO [J].