The ArfGAP Glo3 is required for the generation of COPI vesicles

被引：49

作者：

Lewis, SM

Poon, PP

Singer, RA

Johnston, GC

Spang, A ^{[1
]}

机构：

[1] Max Planck Gesell, Friedrich Miescher Lab, D-72076 Tubingen, Germany

[2] Dalhousie Univ, Dept Microbiol & Immunol, Halifax, NS B3H 1X5, Canada

[3] Dalhousie Univ, Dept Biochem & Mol Biol, Halifax, NS B3H 1X5, Canada

来源：

MOLECULAR BIOLOGY OF THE CELL | 2004年 / 15卷 / 09期

关键词：

D O I：

10.1091/mbc.E04-04-0316

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The small GTPase Arf and coatomer (COPI) are required for the generation of retrograde transport vesicles. Arf activity is regulated by guanine exchange factors (ArfGEF) and GTPase-activating proteins (ArfGAPs). The ArfGAPs Gcs1 and Glo3 provide essential overlapping function for retrograde vesicular transport from the Golgi to the endoplasmic reticulum. We have identified Glo3 as a component of COPI vesicles. Furthermore, we find that a mutant version of the Glo3 protein exerts a negative effect on retrograde transport, even in the presence of the Arf GAP Gcs1. Finally, we present evidence supporting a role for ArfGAP protein in the generation of COPI retrograde transport vesicles.

引用

页码：4064 / 4072

页数：9

共 33 条

[1] ISOLATION AND PHENOTYPIC ANALYSIS OF CONDITIONAL-LETHAL, LINKER-INSERTION MUTATIONS IN THE GENE ENCODING THE LARGEST SUBUNIT OF RNA POLYMERASE-II IN SACCHAROMYCES-CEREVISIAE [J].