Engineering desiccation tolerance in Escherichia coli

被引：66

作者：

Billi, D

Wright, DJ

Helm, RF

Prickett, T

Potts, M

Crowe, JH

机构：

[1] Virginia Polytech Inst & State Univ, VIGEN, Dept Biochem, Fralin Biotechnol Ctr, Blacksburg, VA 24061 USA

[2] Univ Calif Davis, Dept Mol & Cell Biol, Davis, CA 95616 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 04期

关键词：

D O I：

10.1128/AEM.66.4.1680-1684.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recombinant sucrose-6-phosphate synthase (SpsA) was synthesized in Escherichia coli BL21DE3 by using the spsA gene of the cyanobacterium Synechocystis sp. strain PCC 6803. Transformants exhibited a 10,000-fold increase in survival compared to wild-type cells following either freeze-drying, air drying, or desiccation over phosphorus pentoside, The phase transition temperatures and vibration frequencies (P=O stretch) in phospholipids suggested that sucrose maintained membrane fluidity during cell dehydration.

引用

页码：1680 / 1684

页数：5

共 26 条

[1]

BILLI D, CELL MOL RESPONSES S

[2] REVIVAL AND IDENTIFICATION OF BACTERIAL-SPORES IN 25-MILLION-YEAR-OLD TO 40-MILLION-YEAR-OLD DOMINICAN AMBER [J].