S100A1-deficient male mice exhibit increased exploratory activity and reduced anxiety-related responses

被引:18
作者
Ackermann, Gabriele E.
Marenholz, Ingo
Wolfer, David P.
Chan, Wood Yee
Schaefer, Beat
Erne, Paul
Heizmann, Claus W.
机构
[1] Univ Zurich, Dept Pediat, Div Clin Chem & Biochem, CH-8032 Zurich, Switzerland
[2] Max Delbruck Ctr Mol Med, Berlin, Germany
[3] Univ Zurich, Inst Anat, CH-8006 Zurich, Switzerland
[4] Swiss Fed Inst Technol, Dept Biol, Zurich, Switzerland
[5] Chinese Univ Hong Kong, Fac Med, Dept Anat, Hong Kong, Hong Kong, Peoples R China
[6] Univ Zurich, Dept Pediat, Div Oncol, CH-8006 Zurich, Switzerland
[7] Cantonal Hosp Lucerne, Dept Cardiol, Luzern, Switzerland
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 2006年 / 1763卷 / 11期
关键词
S100; mouse; CNS; anxiety; exploration; immunohistochemistry;
D O I
10.1016/j.bbamcr.2006.08.048
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
S100 proteins comprise a family of Ca2+ binding proteins of at least 21 members. They are distinctly expressed in a variety of cell types and tissues and are thought to play unique roles, although they share a high degree of sequence homology and expression overlap. S100A1 is prominently expressed in the heart, where it takes part in Ca2+-cycling. Its role in the central nervous system (CNS) is largely unknown. We have generated S100A1-deficient mice by gene trap mutagenesis to study the involvement of S100A1 in the cytoarchitecture of the brain, in learning and memory, and in avoidance-approach behavior. S100A1 knock out (KO) mice develop well and their brains present with normal morphology. In wild type (Wt) mice, S100A1 protein was found in the hippocampus, cerebral cortex and amygdala, and partially co-localized with the astrocyte marker glial fibrillary acidic protein (GFAP) in the stratum radiatum of the hippocampus. Astrocytes and neurons of S100A1KO rnice did not differ from those of Wt mice regarding shape, distribution and density. In the water maze, S100A1KO mice performed equally well as Wt, implying that S100A1 is not involved in spatial learning and memory. In avoidance-approach tests, predominantly male S100A1 mice showed reduced anxiety-like responses and enhanced explorative activities. We conclude that S100A1 plays a role in modulating innate fear and exploration of novel stimuli. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:1307 / 1319
页数:13
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