Enhancement of RNA cleavage activity of 10-23 DNAzyme by covalently introduced intercalator

被引：43

作者：

Asanuma, Hiroyuki ^{[1
]}

Hayashi, Hiroyuki

Zhao, Jing

Liang, Xingguo

Yamazawa, Akira

Kuramochi, Takeshi

Matsunaga, Daijiro

Aiba, Yuichiro

Kashida, Hiromu

Komiyama, Makoto

机构：

[1] Nagoya Univ, Grad Sch Engn, Dept Mol Design & Engn, Chikusa Ku, Nagoya, Aichi 4648603, Japan

[2] Japan Sci & Technol Agcy, JST, PRESTO, Kawaguchi 3320012, Japan

[3] Univ Tokyo, RCAST, Meguro Ku, Tokyo 1538904, Japan

来源：

CHEMICAL COMMUNICATIONS | 2006年 / 48期

关键词：

D O I：

10.1039/b611078a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

By introducing an intercalator through D-threoninol to the 10-23 DNAzyme at the junction between its catalytic loop and the binding arm, the RNA cleavage activity was greatly improved.

引用

页码：5062 / 5064

页数：3

共 16 条

[1]

Asanuma H, 2001, ANGEW CHEM INT EDIT, V40, P2671, DOI 10.1002/1521-3773(20010716)40:14<2671::AID-ANIE2671>3.0.CO

[2]

2-Z

[3] Optimisation of the 10-23 DNAzyme-substrate pairing interactions enhanced RNA cleavage activity at purine-cytosine target sites [J].

Cairns, MJ ;

King, A ;

Sun, LQ .

NUCLEIC ACIDS RESEARCH, 2003, 31 (11) :2883-2889

[4] Enhanced growth suppression of Philadephia leukemia cells by targeting bcr3/abI2 and VEGF through antisense strategy [J].

Cong, XL ;

Li, B ;

Yang, RC ;

Feng, SZ ;

Chen, SJ ;

Han, ZC .

LEUKEMIA, 2005, 19 (09) :1517-1524

[5] Precise control of RNA cleavage by ribozyme mimics [J].

Daniher, AT ;

Bashkin, JK .

CHEMICAL COMMUNICATIONS, 1998, (10) :1077-1078

[6] The acridine ring selectively intercalated into a DNA helix at various types of abasic sites: Double strand formation and photophysical properties [J].

Fukui, K ;

Tanaka, K .

NUCLEIC ACIDS RESEARCH, 1996, 24 (20) :3962-3967

[7] Alternating hetero H aggregation of different dyes by interstrand stacking from two DNA-dye conjugates [J].

Kashida, H ;

Asanuma, H ;

Komiyama, M .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (47) :6522-6525

[8] Reversible photocontrol of deoxyribozyme-catalyzed RNA cleavage under multiple-turnover conditions [J].

Keiper, Sonja ;

Vyle, Joseph S. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (20) :3306-3309

[9]

KSHIDA H, 2006, CHEM COMMUN, P2768

[10] Light-regulated catalysis by an RNA-cleaving deoxyribozyme [J].

Liu, Y ;

Sen, D .

JOURNAL OF MOLECULAR BIOLOGY, 2004, 341 (04) :887-892

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