Large-scale, high-throughput screening for coagulation and hematologic phenotypes in mice

被引:73
作者
Peters, LL
Cheever, EM
Ellis, HR
Magnani, PA
Svenson, KL
Von Smith, R
Bogue, MA
机构
[1] Jackson Lab, Bar Harbor, ME 04609 USA
[2] Dade Behring Inc, Deerfield, IL USA
关键词
hemostasis; hematology; mouse phenome; NHLBI program for genomic applications;
D O I
10.1152/physiolgenomics.00077.2002
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The Mouse Phenome Project is an international effort to systematically gather phenotypic data for a defined set of inbred mouse strains. For such large-scale projects the development of high-throughput screening protocols that allow multiple tests to be performed on a single mouse is essential. Here we report hematologic and coagulation data for more than 30 inbred strains. Complete blood counts were performed using an Advia 120 analyzer. For coagulation testing, we successfully adapted the Dade Behring BCS automated coagulation analyzer for use in mice by lowering sample and reagent volume requirements. Seven automated assay procedures were developed. Small sample volume requirements make it possible to perform multiple tests on a single animal without euthanasia, while reductions in reagent volume requirements reduce costs. The data show that considerable variation in many basic hematological and coagulation parameters exists among the inbred strains. These data, freely available on the World Wide Web, allow investigators to knowledgeably select the most appropriate strain(s) to meet their individual study designs and goals.
引用
收藏
页码:185 / 193
页数:9
相关论文
共 11 条
[1]  
CLARK AT, IN PRESS GENETICA
[2]   A mouse model of severe von Willebrand disease:: Defects in hemostasis and thrombosis [J].
Denis, C ;
Methia, N ;
Frenette, PS ;
Rayburn, H ;
Ullman-Culleré, M ;
Hynes, RO ;
Wagner, DD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (16) :9524-9529
[3]  
Fleming MD, 1997, NAT GENET, V16, P383, DOI 10.1038/ng0897-383
[4]   Inactivation of the gene for anticoagulant protein C causes lethal perinatal consumptive coagulopathy in mice [J].
Jalbert, LR ;
Rosen, ED ;
Moons, L ;
Chan, JCY ;
Carmeliet, P ;
Collen, D ;
Castellino, FJ .
JOURNAL OF CLINICAL INVESTIGATION, 1998, 102 (08) :1481-1488
[5]   The molecular basis of the obese mutation in ob(2J) mice [J].
Moon, BC ;
Friedman, JM .
GENOMICS, 1997, 42 (01) :152-156
[6]   A mouse phenome project [J].
Paigen, K ;
Eppig, JT .
MAMMALIAN GENOME, 2000, 11 (09) :715-717
[7]  
Paigen Kenneth, 2002, ILAR J, V43, P123
[8]  
PETERS LL, 2001, MPD62 JACKS LAB
[9]   Concordance of murine quantitative trait loci for salt-induced hypertension with rat and human loci [J].
Sugiyama, F ;
Churchill, GA ;
Higgins, DC ;
Johns, C ;
Makaritsis, KP ;
Gavras, H ;
Paigen, B .
GENOMICS, 2001, 71 (01) :70-77
[10]   Hemostasis in the mouse (Mus musculus):: A review [J].
Tsakiris, DA ;
Scudder, L ;
Hodivala-Dilke, K ;
Hynes, RO ;
Coller, BS .
THROMBOSIS AND HAEMOSTASIS, 1999, 81 (02) :177-188