MALDI-TOF MS-based drug susceptibility testing of pathogens: The example of Candida albicans and fluconazole

被引：93

作者：

Marinach, Carine ^{[2
]}

Alanio, Alexandre ^{[2
,3
]}

Palous, Martine ^{[3
]}

Kwasek, Stephanie ^{[2
]}

Fekkar, Arnaud ^{[2
,3
]}

Brossas, Jean-Yves ^{[2
,4
]}

Brun, Sophie ^{[2
,3
]}

Snounou, Georges ^{[2
,5
]}

Hennequin, Christophe ^{[2
]}

Sanglard, Dominique ^{[6
,7
]}

Datry, Annick ^{[3
]}

Golmard, Jean-Louis ^{[8
]}

Mazier, Dominique ^{[1
,2
,3
]}

机构：

[1] UPMC, Grp Hosp Pitie Salpetriere, INSERM, UMRS 945, F-75013 Paris, France

[2] Univ Paris 06, Paris, France

[3] Grp Hosp Pitie Salpetriere, AP HP, Serv Parasitol Mycol, F-75634 Paris, France

[4] Grp Hosp Pitie Salpetriere, Ctr Invest Biomed, F-75634 Paris, France

[5] Natl Univ Singapore, Dept Microbiol, Singapore 117548, Singapore

[6] Univ Lausanne, Inst Microbiol, Lausanne, Switzerland

[7] Univ Hosp Ctr, Lausanne, Switzerland

[8] Grp Hosp Pitie Salpetriere, Modelisat Rech Clin, EA 3974, F-75634 Paris, France

来源：

PROTEOMICS | 2009年 / 9卷 / 20期

关键词：

Algorithm; Candida albicans; MALDI-TOF MS; Microbiology; Proteome profiling; Target for drugs; MASS-SPECTROMETRY; INTERPRETIVE BREAKPOINTS; EUROPEAN COMMITTEE; NATIONAL COMMITTEE; CLSI M27-A2; IDENTIFICATION; ITRACONAZOLE; INFECTIONS; SPP;

D O I：

10.1002/pmic.200900152

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

MALDI-TOF MS can be used for the identification of microorganism species. We have extended its application to a novel assay of Candida albicans susceptibility to fluconazole, based on monitoring modifications of the proteome of yeast cells grown in the presence of varying drug concentrations. The method was accurate, and reliable, and showed full agreement with the Clinical Laboratory Standards Institute's reference method. This proof-of-concept demonstration highlights the potential for this approach to test other pathogens.

引用

页码：4627 / 4631

页数：5

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