Spatio-temporal analysis of mitotic activity with a labile cyclin-GUS fusion protein

被引：581

作者：

Colón-Carmona, A ^{[1
]}

You, R ^{[1
]}

Haimovitch-Gal, T ^{[1
]}

Doerner, P ^{[1
]}

机构：

[1] Salk Inst Biol Studies, Plant Biol Lab, La Jolla, CA 92037 USA

来源：

PLANT JOURNAL | 1999年 / 20卷 / 04期

关键词：

D O I：

10.1046/j.1365-313x.1999.00620.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Plant growth responds rapidly to developmental and environmental signals, but the underlying changes in cell division activity are poorly understood. A labile cyclin-GUS reporter was developed to facilitate the spatio-temporal analysis of cell division patterns. The chimeric reporter protein is turned over every cell cycle and hence its histochemical activity accurately reports individual mitotic cells. Using Arabidopsis plants transformed with cyclin-GUS, we visualized patterns of mitotic activity in wounded leaves which suggest a role for cell division in structural reinforcement.

引用

页码：503 / 508

页数：6

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