Nitrate reductase for nitrate analysis in water

被引：23

作者：

Campbell, Wilbur H.

Song, Pengfei

Barbier, Guillaume G.

机构：

[1] Nitrate Eliminat Co Inc, Lake Linden, MI 49945 USA

[2] Michigan Technol Univ, Dept Biol Sci, Houghton, MI 49931 USA

来源：

ENVIRONMENTAL CHEMISTRY LETTERS | 2006年 / 4卷 / 02期

关键词：

nitrate reductase; recombinant expression; nitrate; Green chemistry; water; enzymes;

D O I：

10.1007/s10311-006-0035-4

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Nitrate analysis in water is one of the most frequently applied methods in environmental chemistry. Current methods for nitrate are generally based on toxic substances. Here, we show that a viable alternative method is to use the enzyme nitrate reductase. The key to applying this Green Chemistry solution for nitrate analysis is plentiful, inexpensive, analytical grade enzyme. We demonstrate that recombinant Arabidopsis nitrate reductase, expressed in the methylotrophic yeast Pichia pastoris, is a highly effective catalyst for nitrate analysis at 37 degrees C. Recombinant production of enzyme ensures consistent quality and provides means to meet the needs of environmental chemistry.

引用

收藏

页码：69 / 73

页数：5

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