Nanoscale resolution in GFP-based microscopy

被引：256

作者：

Willig, Katrin I. ^{[1
]}

Kellner, Robert R. ^{[1
]}

Medda, Rebecca ^{[1
]}

Hein, Birka ^{[1
]}

Jakobs, Stefan ^{[1
]}

Hell, Stefan W. ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept NanoBiophton, D-37077 Gottingen, Germany

来源：

NATURE METHODS | 2006年 / 3卷 / 09期

关键词：

D O I：

10.1038/NMETH922

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The similar to 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.

引用

页码：721 / 723

页数：3

共 15 条

[1] A monomeric red fluorescent protein
Campbell, RE
Tour, O
Palmer, AE
Steinbach, PA
Baird, GS
Zacharias, DA
Tsien, RY
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (12) : 7877 - 7882
[2] Individual rotavirus-like particles containing 120 molecules of fluorescent protein are visible in living cells
Charpilienne, A
Nejmeddine, M
Berois, M
Parez, N
Neumann, E
Hewat, E
Trugnan, G
Cohen, J
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (31) : 29361 - 29367
[3] Optical sectioning microscopy
Conchello, JA
Lichtman, JW
[J]. NATURE METHODS, 2005, 2 (12) : 920 - 931
[4] Immunofluorescence stimulated emission depletion microscopy
Dyba, M
Jakobs, S
Hell, SW
[J]. NATURE BIOTECHNOLOGY, 2003, 21 (11) : 1303 - 1304
[5] Reducing the environmental sensitivity of yellow fluorescent protein - Mechanism and applications
Griesbeck, O
Baird, GS
Campbell, RE
Zacharias, DA
Tsien, RY
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (31) : 29188 - 29194
[6] Toward fluorescence nanoscopy
Hell, SW
[J]. NATURE BIOTECHNOLOGY, 2003, 21 (11) : 1347 - 1355
[7] BREAKING THE DIFFRACTION RESOLUTION LIMIT BY STIMULATED-EMISSION - STIMULATED-EMISSION-DEPLETION FLUORESCENCE MICROSCOPY
HELL, SW
WICHMANN, J
[J]. OPTICS LETTERS, 1994, 19 (11) : 780 - 782
[8] Bruchpilot promotes active zone assembly, Ca2+ channel clustering, and vesicle release
Kittel, RJ
Wichmann, C
Rasse, TM
Fouquet, W
Schmidt, M
Schmid, A
Wagh, DA
Pawlu, C
Kellner, RR
Willig, KI
Hell, SW
Buchner, E
Heckmann, M
Sigrist, SJ
[J]. SCIENCE, 2006, 312 (5776) : 1051 - 1054
[9] Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission
Klar, TA
Jakobs, S
Dyba, M
Egner, A
Hell, SW
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (15) : 8206 - 8210
[10] PAWLEY JB, 2006, HDB BIOL CONFOCAL MI

← 1 2 →