Changes in messenger RNA abundance of amino acid transporters in rat mammary gland during pregnancy, lactation, and weaning

被引:33
作者
Aleman, Gabriela [1 ]
Lopez, Adriana [1 ]
Ordaz, Guillermo [1 ]
Torres, Nimbe [1 ]
Tovar, Armando R. [1 ]
机构
[1] Inst Nacl Ciencias Med & Nutr Salvador Zubiran, Dept Fisiol Nutr, Mexico City 14000, DF, Mexico
来源
METABOLISM-CLINICAL AND EXPERIMENTAL | 2009年 / 58卷 / 05期
关键词
DAM RAT; EXPRESSION; METABOLISM; TISSUE; TAURINE; MOUSE; LIVER; GLUTAMINE; PROTEINS; ALANINE;
D O I
10.1016/j.metabol.2008.12.003
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
During lactation, the mammary gland increases the needs for nutrients to fulfill the milk production requirements. Among these nutrients, amino acids play an important role for the synthesis of milk proteins. Amino acids are supplied to the mammary gland through amino acid transporters, although sonic arc synthesized in situ. The purpose of this study was to establish the pattern of changes in messenger RNA abundance of the amino acid transporters ASC. system L, EAAC 1, GLAST, CAT-1 and Tau in the mammary gland of the rat during different stages of pregnancy and lactation. Rats were fed during pregnancy and lactation a 20% casein diet. Food intake increased significantly during the lactation period. Amino acid transporter ASC expression increased during the first days of pregnancy about 2-fold, and it was increased in a lesser extent again during the peak of lactation. The expression of system L (LAT-1) and CAT-1 transporters was increased only during the lactation period. On the other hand, the expression of the transporters for anionic amino acids EAAC1 and GLAST was low during both stages. Finally, taurine transporter expression decreased during pregnancy; and it was significantly lower during lactation. These results showed that amino acid transporters were not expressed similarly in the mammary gland during pregnancy and lactation, indicating that the expression of these transporters did not respond only to the metabolic needs of the gland but depended on the dietary protein supply and possibly the specific hormonal changes that occur during pregnancy and lactation. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码:594 / 601
页数:8
相关论文
共 50 条
  • [1] ARRIZA JL, 1993, J BIOL CHEM, V268, P15329
  • [2] Elevated expression of liver γ-cystathionase is required for the maintenance of lactation in rats
    Barber, T
    Triguero, A
    Martínez-López, I
    Torres, L
    García, C
    Miralles, VJ
    Viña, JR
    [J]. JOURNAL OF NUTRITION, 1999, 129 (05) : 928 - 933
  • [3] CATIONIC AMINO-ACID-TRANSPORT BY BOVINE MAMMARY TISSUE
    BAUMRUCKER, CR
    [J]. JOURNAL OF DAIRY SCIENCE, 1984, 67 (11) : 2500 - 2506
  • [4] REGULATION OF ORGANIC NUTRIENT METABOLISM DURING TRANSITION FORM LATE PREGNANCY TO EARLY LACTATION
    BELL, AW
    [J]. JOURNAL OF ANIMAL SCIENCE, 1995, 73 (09) : 2804 - 2819
  • [5] Recent molecular advances in mammalian glutamine transport
    Bode, BP
    [J]. JOURNAL OF NUTRITION, 2001, 131 (09) : 2475S - 2485S
  • [6] Evidence for an interaction between cationic and neutral amino acids at the blood-facing aspect of the lactating rat mammary epithelium
    Calvert, DT
    Shennan, DB
    [J]. JOURNAL OF DAIRY RESEARCH, 1996, 63 (01) : 25 - 33
  • [7] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
  • [8] Structure and function of cationic amino acid transporters (CATs)
    Closs, E. I.
    Boissel, J.-P.
    Habermeier, A.
    Rotmann, A.
    [J]. JOURNAL OF MEMBRANE BIOLOGY, 2006, 213 (02) : 67 - 77
  • [9] Regulation of branched-chain amino acid metabolism in the lactating rat
    DeSantiago, S
    Torres, N
    Suryawan, A
    Tovar, AR
    Hutson, SM
    [J]. JOURNAL OF NUTRITION, 1998, 128 (07) : 1165 - 1171
  • [10] DeSantiago S, 1996, ARCH MED RES, V27, P443